Document Detail

Effect of chronic intermittent hypoxia on biological behavior and hypoxia-associated gene expression in lung cancer cells.
MedLine Citation:
PMID:  20568121     Owner:  NLM     Status:  MEDLINE    
Hypoxia plays an important role in the development of solid tumors and is associated with their therapeutic resistance. There exist three major forms of hypoxia: acute, chronic, and intermittent hypoxia. Previous studies have shown that cancer cells could behave in the form of adaptation to hypoxia in tumor growth, which could result in their biological changes and determine their responses to the therapies. To investigate the tumor cells' adaptation to hypoxia, we recreated two models using two lung cancer cell lines in the presence of intermittent hypoxia, which is characterized by changes in oxygen pressure within the disorganized vascular network. We investigated biological behaviors such as cell cycle, proliferation, radiation sensitivity, apoptosis and migration, hypoxia signal pathway in the lung cancer cells treated with chronic intermittent hypoxia, as well as the role of hypoxia inducible factor 1 there, hypoxia-inducible genes analyzed by real-time RT-PCR chip in H446 cells treated with the model. The results indicated the changes of some hypoxia target gene expressions of those induced by hypoxia, some of which were confirmed by real-time RT-PCR. The cells mediated by irradiation induced resistance to radiation and apoptosis and increased metastasis in lung cancer cells. It was found that such changes were related to hypoxia inducible factor 1, alpha subunit (HIF-1α).
Yonglei Liu; Xianrang Song; Xingwu Wang; Ling Wei; Xiuju Liu; Shuanghu Yuan; Liyan Lv
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  111     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  554-63     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Cancer Research Center, Shandong Tumor Hospital, Jinan, Shandong Province, PR China.
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MeSH Terms
Anoxia / genetics*
Cell Line, Tumor
Dose-Response Relationship, Radiation
Gene Expression / radiation effects
Hypoxia-Inducible Factor 1, alpha Subunit
Lung Neoplasms / pathology*
Neoplasm Metastasis
Reg. No./Substance:
0/Hypoxia-Inducible Factor 1, alpha Subunit

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