Document Detail

Effect of chronic high altitude hypoxia on foetal and maternal juxta-alveolar distal pulmonary smooth muscle cells actin and calponin organisation and growth profiles.
MedLine Citation:
PMID:  21465446     Owner:  NLM     Status:  In-Data-Review    
BACKGROUND: The effect of chronic high altitude hypoxia (CHAH) in the juxta-alveolar region near the air-blood interface is unknown because of the experimental inaccessibility of this region.
OBJECTIVE: To examine primary cultures of digested juxtaalveolar smooth muscle cells for hypoxia-induced changes.
METHODS: Smooth Muscle Cells (SMCs) obtained by dispase digestion of the extreme lung parenchyma were used to study the effect of CHAH in the juxta-alveolar region and foetal and maternal cells were compared. Pulmonary venous SMCs were also obtained from dissected 5th to 7th generation levels pulmonary veins (<0.5 mm). Fluorescence tagged antibodies against alpha smooth muscle actin (alpha SMA) and calponin respectively were used as markers to identify cellular structural differences by routine immunohistochemistry. Comparison of the functional integrity of the cells was made using their growth profiles obtained by radiolabeled thymidine incorporation and liquid scintillation counting.
RESULTS: Marked differences were seen in juxta-alveolar SMCs obtained by digestion of extreme lung parenchyma of hypoxic sheep. Hypoxic adult sheep cells showed increased filamentation. Hypoxic foetal sheep cells showed internal restructuring and disorganization of both alpha-SMA and calponin filaments. The growth profiles of juxta-alveolar SMCs showed that the hypoxia-affected cells of both the foetus and adult sheep had a fast initial growth rate peaking at 48h while their normoxic equivalents had a steadier growth rate peaking at 72h. Hypoxia-affected cells showed contact inhibition at ~50% subconfluence and apoptosis by 48h.
CONCLUSION: Chronic high altitude hypoxia causes both phenotypical and functional changes in pulmonary smooth muscle cells near the air/blood interface.
T A John
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  West African journal of medicine     Volume:  29     ISSN:  0189-160X     ISO Abbreviation:  West Afr J Med     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2011-04-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8301891     Medline TA:  West Afr J Med     Country:  Nigeria    
Other Details:
Languages:  eng     Pagination:  388-92     Citation Subset:  IM    
T. A. John, Department of Pharmacology, Lagos State University College of Medicine, PMB 21266, Ikeja, Lagos. Email: Tel: 234 7028338910.
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