Document Detail

Effect of chronic excess of tumour necrosis factor-alpha on contractile proteins in rat skeletal muscle.
MedLine Citation:
PMID:  11086712     Owner:  NLM     Status:  MEDLINE    
The effect of chronic tumour necrosis factor-alpha (TNF-alpha) treatment on the synthesis of specific myofibrillar proteins such as heavy chain myosin, light chain myosin and G-actin in rat diaphragm were evaluated. Muscles (diaphragm) from control and experimental groups (TNF-alpha i.v. at 50 microg/kg body wt for 5 days) were incubated in the presence of 35S-methionine for 2 h. Myofibrillar protein extracts were prepared and protein was electrophoresed on sodium dodecyl sulphate-polyacrylamide gels. Heavy chain myosin, light chain myosin and G-actin were identified by Western blot analysis using specific monoclonal antibodies. Polyacrylamide gel electrophoresis (PAGE) followed by Western blot analysis revealed two types of heavy chain myosin (206 and 212 kD), all four types of light chain myosin (15, 16.5, 18 and 20 kD) and a single type of G-actin (42 kD). Chronic TNF-alpha treatment produced a significant decline in the synthesis of all types of myofibrillar proteins, namely heavy chain myosin, light chain myosin and G-actin. TNF-alpha impaired peptide-chain initiation in diaphragm muscle which was reversed by the branched-chain amino acids (BCAA) therapy of TNF-alpha treated rats. These findings indicate a significant role for TNF-alpha in the translational regulation of protein synthesis in skeletal muscle.
I R Cheema; C Hermann; S Postell; P Barnes
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytobios     Volume:  103     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  2000  
Date Detail:
Created Date:  2001-02-09     Completed Date:  2001-03-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA.
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MeSH Terms
Actins / biosynthesis,  drug effects,  ultrastructure
Amino Acids, Branched-Chain / pharmacology
Contractile Proteins / biosynthesis*,  drug effects*,  ultrastructure
Drug Administration Schedule
Muscle, Skeletal / drug effects*,  metabolism*,  ultrastructure
Myosin Heavy Chains / biosynthesis,  drug effects,  ultrastructure
Myosin Light Chains / biosynthesis,  drug effects,  ultrastructure
Rats, Wistar
Tumor Necrosis Factor-alpha / metabolism,  pharmacology*
Grant Support
Reg. No./Substance:
0/Actins; 0/Amino Acids, Branched-Chain; 0/Contractile Proteins; 0/Myosin Heavy Chains; 0/Myosin Light Chains; 0/Tumor Necrosis Factor-alpha

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