Document Detail

Effect of cholesterol on the physical state and function of lymphocyte membranes
MedLine Citation:
PMID:  3006795     Owner:  NLM     Status:  MEDLINE    
Effect of gradual increase of cholesterol content in T-lymphocyte membranes on the structure and physical state of plasmic membrane lipids and activities of the membrane-bound enzymes was investigated. The increase in cholesterol content was shown to result in a two-phase change of luminescence parameters of the fluorescent probes dimethylaminochalcone and pyrene, which indicates heterogeneity of cholesterol in the membranes. With the growth of steroid content in the cell membranes, at first, we observed a sharp decrease in the lipid bilayer fluidity and inhibition of Na+, K+-ATPase activity, which at the molar ratio cholesterol/phospholipids 0.6 in thymocyte membranes, remains at the same level. With higher cholesterol concentrations ATPase activity did not change. The effect of cholesterol on ATPase activity was in a good agreement with the effect of membrane lipids on fluidity. It is suggested that two pools of cholesterol molecules exist in the membranes, differing in their effects of bilayer fluidity and functional activity of the membranes.
G I Klebanov; L I Ivanova; E M Turkmenova; Iu A Vladimirov
Publication Detail:
Type:  English Abstract; In Vitro; Journal Article    
Journal Detail:
Title:  Biofizika     Volume:  31     ISSN:  0006-3029     ISO Abbreviation:  Biofizika     Publication Date:    1986 Jan-Feb
Date Detail:
Created Date:  1986-04-30     Completed Date:  1986-04-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372666     Medline TA:  Biofizika     Country:  USSR    
Other Details:
Languages:  rus     Pagination:  73-8     Citation Subset:  IM    
Vernacular Title:
Vliianie kholesterina na fizicheskoe sostoianie i funktsionirovanie membran limfotsitov.
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MeSH Terms
Ca(2+) Mg(2+)-ATPase / metabolism
Cell Membrane / enzymology,  metabolism
Cholesterol / metabolism*
Membrane Lipids / metabolism*
Sodium-Potassium-Exchanging ATPase / metabolism
T-Lymphocytes / enzymology,  metabolism*
Reg. No./Substance:
0/Liposomes; 0/Membrane Lipids; 57-88-5/Cholesterol; EC 3.6.1.-/Ca(2+) Mg(2+)-ATPase; EC ATPase

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