| Effect of cholesterol and fatty acids on the molecular interactions of fengycin with Stratum corneum mimicking lipid monolayers. | |
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MedLine Citation:
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PMID: 19437771 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The combination of atomic force microscopy (AFM) and the Langmuir trough technique was used in this work to investigate the molecular interactions of fengycin with lipid monolayers constituted of the major lipid classes found in human stratum corneum (SC). AFM imaging o f spread SC lipids/fengycin monolayers showed that fengycin preferentially partitions into cholesterol-rich phases surrounding 2D domains mainly constituted of ceramide and fatty acid molecules. Penetration experiments of fengycin from the subphase into SC-mimicking monolayers clearly indicated that the lipopeptide insertion at the lipid interface is enhanced in the presence of cholesterol. AFM analysis of mixed SC lipids/fengycin monolayers obtained after lipopeptide penetration revealed that cholesterol strongly interacts with fengycin and undergoes specific molecular interactions with more disordered, loosely packed ceramide molecules. These results highlight the capacity of fengycin to interact with the lipid constituents of the extracellular matrix of SC and, in particular, with cholesterol. |
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Authors:
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M Eeman; G Francius; Y F Dufrêne; K Nott; M Paquot; M Deleu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 25 ISSN: 0743-7463 ISO Abbreviation: Langmuir Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-05-13 Completed Date: 2009-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 3029-39 Citation Subset: IM |
Affiliation:
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Unité de Chimie Biologique Industrielle, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, B-5030 Gembloux, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ceramides
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chemistry Cholesterol / chemistry* Drug Design Epidermis / metabolism Extracellular Matrix / metabolism Fatty Acids / chemistry* Lipids / chemistry* Lipopeptides / chemistry*, pharmacology Microscopy, Atomic Force / methods* Models, Chemical Models, Statistical Protein Structure, Tertiary Skin / drug effects*, metabolism*, microbiology* Surface Properties Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Ceramides; 0/Fatty Acids; 0/Lipids; 0/Lipopeptides; 102577-03-7/fengycin; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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