Document Detail

Effect of chlorophyllin on normothermic storage of human periodontal ligament cells.
MedLine Citation:
PMID:  15167465     Owner:  NLM     Status:  MEDLINE    
The purpose of the present study was to evaluate whether chlorophyllin could serve as an effective constituent of a storage medium to enhance the human periodontal ligament (PDL) cell viability. Freshly isolated PDL cells from premolars extracted from healthy people were stored at 37 degrees C for 6 h in various solutions: F-medium and Hank's balanced salt solution (HBSS), supplemented with chlorophyllin. From MTT viability assays, the highest cell viability was found in the PDL cells stored in HBSS supplemented with 500 nM chlorophyllin, and the chlorophyllin-treated cells showed a dose-dependent response to concentration. Additionally, the results from flow cytometry showed that 77 to 80% of the PDL cells were in the G0/G1 phases of the cell cycle, which suggested that most were in a stable stage. These result showed that HBSS, supplemented with chlorophyllin, may be a useful solution for preserving the viability of PDL cells.
Won-Gyun Chung; Eun Ju Lee; Seung-Jong Lee; Seung-Ae Lee; Jin Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endodontics     Volume:  30     ISSN:  0099-2399     ISO Abbreviation:  J Endod     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-28     Completed Date:  2004-08-12     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  399-402     Citation Subset:  D    
Department of Dental Hygiene, Yonsei University, Wonju College of Medicine, Wonju-City, Kangwon-Do, Korea.
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MeSH Terms
Antioxidants / pharmacology*
Cell Survival / drug effects
Cells, Cultured
Chlorophyllides / pharmacology*
Coloring Agents / diagnostic use
Culture Media
Dose-Response Relationship, Drug
Flow Cytometry
G0 Phase
G1 Phase
Isotonic Solutions / pharmacology
Periodontal Ligament / cytology,  drug effects*
Tetrazolium Salts / diagnostic use
Thiazoles / diagnostic use
Tissue Preservation*
Reg. No./Substance:
0/Antioxidants; 0/Chlorophyllides; 0/Coloring Agents; 0/Culture Media; 0/Hanks Balanced Salt Solution; 0/Isotonic Solutions; 0/Tetrazolium Salts; 0/Thiazoles; 15611-43-5/chlorophyllin; 298-93-1/thiazolyl blue

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