| Effect of a chloride channel inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate, on ovarian cancer cell migration. | |
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MedLine Citation:
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PMID: 21888019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Chloride channels (ClC) are involved in normal physiological processes and pathology of various diseases. Although it is recognized that suppression of ClC inhibits cell proliferation in different types of cells, the potential function of ClC in cell migration in ovarian cancer is still unclear. In this study, we investigated the effect of the ClC inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), on cell migration in the human ovarian cancer cell line SKOV-3 as well as the related signaling pathway involved in this action. METHODS: In this study, cell viability was measured using the MTT assay. Transwell migration method was used to study the effect of NPPB on serum-induced SKOV-3 cell migration. Also, Western blot was performed to detect the phosphorylation levels of ERK1/2 and AKT1 after treatment with NPPB. RESULTS: Both NPPB and LY249002 significantly inhibited serum-induced SKOV-3 cell migration without alteration of cell viability. NPPB's inhibition of phosphorylation of AKT1 was time-dependent (p < 0.05). There was no significant effect on the phosphorylation of ERK1/2 after treatment with NPPB. CONCLUSIONS: ClC plays an important role in ovarian cancer cell migration. NPPB inhibited-SKOV-3 cell migration could be via inactivation of AKT1. |
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Authors:
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Zhan Yu; Zhi-Xu Zhang; Shenglei Li; Jianbo Gao |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinical laboratory Volume: 57 ISSN: 1433-6510 ISO Abbreviation: Clin. Lab. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-09-05 Completed Date: 2011-10-20 Revised Date: 2012-06-22 |
Medline Journal Info:
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Nlm Unique ID: 9705611 Medline TA: Clin Lab Country: Germany |
Other Details:
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Languages: eng Pagination: 543-50 Citation Subset: IM |
Affiliation:
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Department of Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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enzymology,
pathology* Angiogenesis Inhibitors / pharmacology Cell Line, Tumor / cytology, drug effects, enzymology Cell Movement / drug effects Chloride Channels / antagonists & inhibitors*, physiology Culture Media / pharmacology Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Female Flavonoids / pharmacology Humans MAP Kinase Signaling System / drug effects Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors, physiology Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors, physiology Nitrobenzoates / pharmacology* Ovarian Neoplasms / enzymology, pathology* Phosphatidylinositol 3-Kinases / antagonists & inhibitors, physiology Protein Kinase Inhibitors / pharmacology Proto-Oncogene Proteins c-akt / physiology Serum Signal Transduction / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inhibitors; 0/Chloride Channels; 0/Culture Media; 0/Flavonoids; 0/Nitrobenzoates; 0/PD 98059; 0/Protein Kinase Inhibitors; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.11.1/AKT1 protein, human; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/MAPK1 protein, human; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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