Document Detail

Effect of a chloride channel inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate, on ovarian cancer cell migration.
MedLine Citation:
PMID:  21888019     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Chloride channels (ClC) are involved in normal physiological processes and pathology of various diseases. Although it is recognized that suppression of ClC inhibits cell proliferation in different types of cells, the potential function of ClC in cell migration in ovarian cancer is still unclear. In this study, we investigated the effect of the ClC inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), on cell migration in the human ovarian cancer cell line SKOV-3 as well as the related signaling pathway involved in this action.
METHODS: In this study, cell viability was measured using the MTT assay. Transwell migration method was used to study the effect of NPPB on serum-induced SKOV-3 cell migration. Also, Western blot was performed to detect the phosphorylation levels of ERK1/2 and AKT1 after treatment with NPPB.
RESULTS: Both NPPB and LY249002 significantly inhibited serum-induced SKOV-3 cell migration without alteration of cell viability. NPPB's inhibition of phosphorylation of AKT1 was time-dependent (p < 0.05). There was no significant effect on the phosphorylation of ERK1/2 after treatment with NPPB.
CONCLUSIONS: ClC plays an important role in ovarian cancer cell migration. NPPB inhibited-SKOV-3 cell migration could be via inactivation of AKT1.
Zhan Yu; Zhi-Xu Zhang; Shenglei Li; Jianbo Gao
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical laboratory     Volume:  57     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-05     Completed Date:  2011-10-20     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  543-50     Citation Subset:  IM    
Department of Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China.
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MeSH Terms
Adenocarcinoma / enzymology,  pathology*
Angiogenesis Inhibitors / pharmacology
Cell Line, Tumor / cytology,  drug effects,  enzymology
Cell Movement / drug effects
Chloride Channels / antagonists & inhibitors*,  physiology
Culture Media / pharmacology
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Flavonoids / pharmacology
MAP Kinase Signaling System / drug effects
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors,  physiology
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors,  physiology
Nitrobenzoates / pharmacology*
Ovarian Neoplasms / enzymology,  pathology*
Phosphatidylinositol 3-Kinases / antagonists & inhibitors,  physiology
Protein Kinase Inhibitors / pharmacology
Proto-Oncogene Proteins c-akt / physiology
Signal Transduction / drug effects*
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Angiogenesis Inhibitors; 0/Chloride Channels; 0/Culture Media; 0/Flavonoids; 0/Nitrobenzoates; 0/Protein Kinase Inhibitors; 3A35O9G3YZ/5-nitro-2-(3-phenylpropylamino)benzoic acid; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC protein, human; EC Proteins c-akt; EC protein, human; EC Protein Kinase 1; EC Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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