Document Detail


Effect of a chloride channel inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate, on ovarian cancer cell migration.
MedLine Citation:
PMID:  21888019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Chloride channels (ClC) are involved in normal physiological processes and pathology of various diseases. Although it is recognized that suppression of ClC inhibits cell proliferation in different types of cells, the potential function of ClC in cell migration in ovarian cancer is still unclear. In this study, we investigated the effect of the ClC inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), on cell migration in the human ovarian cancer cell line SKOV-3 as well as the related signaling pathway involved in this action.
METHODS: In this study, cell viability was measured using the MTT assay. Transwell migration method was used to study the effect of NPPB on serum-induced SKOV-3 cell migration. Also, Western blot was performed to detect the phosphorylation levels of ERK1/2 and AKT1 after treatment with NPPB.
RESULTS: Both NPPB and LY249002 significantly inhibited serum-induced SKOV-3 cell migration without alteration of cell viability. NPPB's inhibition of phosphorylation of AKT1 was time-dependent (p < 0.05). There was no significant effect on the phosphorylation of ERK1/2 after treatment with NPPB.
CONCLUSIONS: ClC plays an important role in ovarian cancer cell migration. NPPB inhibited-SKOV-3 cell migration could be via inactivation of AKT1.
Authors:
Zhan Yu; Zhi-Xu Zhang; Shenglei Li; Jianbo Gao
Related Documents :
22266309 - Activation of p53-p21 is closely associated with the acquisition of resistance to apopt...
22096139 - Inhibitory effect of corneal endothelial cells on il-17-producing th17 cells.
21895799 - Split decision: a thaumarchaeon encoding both ftsz and cdv cell division proteins choos...
16648849 - Distinct timing in the activity of cannabinoid-sensitive and cannabinoid-insensitive ba...
3813769 - Increased efficacy of antidromic and orthodromic activation of cat alpha motoneurons up...
21904979 - Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells.
19293989 - Phenotype analysis and quantification of proliferating cells in the cortical gray matte...
11228229 - Distribution of cyclooxygenase-2 in eutopic and ectopic endometrium in endometriosis an...
20331609 - Glycosidated phospholipids: uncoupling of signalling pathways at the plasma membrane.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical laboratory     Volume:  57     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-05     Completed Date:  2011-10-20     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  543-50     Citation Subset:  IM    
Affiliation:
Department of Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology,  pathology*
Angiogenesis Inhibitors / pharmacology
Cell Line, Tumor / cytology,  drug effects,  enzymology
Cell Movement / drug effects
Chloride Channels / antagonists & inhibitors*,  physiology
Culture Media / pharmacology
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Female
Flavonoids / pharmacology
Humans
MAP Kinase Signaling System / drug effects
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors,  physiology
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors,  physiology
Nitrobenzoates / pharmacology*
Ovarian Neoplasms / enzymology,  pathology*
Phosphatidylinositol 3-Kinases / antagonists & inhibitors,  physiology
Protein Kinase Inhibitors / pharmacology
Proto-Oncogene Proteins c-akt / physiology
Serum
Signal Transduction / drug effects*
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Angiogenesis Inhibitors; 0/Chloride Channels; 0/Culture Media; 0/Flavonoids; 0/Nitrobenzoates; 0/Protein Kinase Inhibitors; 3A35O9G3YZ/5-nitro-2-(3-phenylpropylamino)benzoic acid; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.11.1/AKT1 protein, human; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/MAPK1 protein, human; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cathepsin B, D, and L regulation in cyclosporin A-mediated gingival hyperplasia of a patient with sa...
Next Document:  Lipid peroxidation and parathyroid hormone influence the cytosolic calcium levels of erythrocytes in...