| Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter. | |
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MedLine Citation:
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PMID: 9435484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H(+)-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter, PO2 required for half-maximal activity was 37 +/- 3 mmHg (4.9 kPa). Although RBCs were swollen at low pH, results showed explicitly that the volume change per se had little effect on K influx. Lactate had no effect on volume- or H(+)-stimulated K influxes, nor did bicarbonate or PCO2 affect the magnitude of K influxes after these stimuli or after treatment with protein kinase/phosphatase inhibitors. These results represent the first detailed report of O2 dependence of H(+)-stimulated K-Cl cotransport in RBCs from any mammalian species. They emphasize the importance of PO2 in control of RBC K-Cl cotransport. |
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Authors:
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P F Speake; C A Roberts; J S Gibson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 273 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-02-09 Completed Date: 1998-02-09 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C1811-8 Citation Subset: IM |
Affiliation:
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Department of Veterinary Preclinical Sciences, University of Liverpool, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bicarbonates / blood Body Water Carbon Dioxide / blood Carrier Proteins / blood* Erythrocytes / metabolism* Hematocrit Horses / physiology* Hydrogen-Ion Concentration Lactates / blood Oxygen / blood* Partial Pressure Physical Exertion / physiology* Potassium / blood* Respiration Rubidium Radioisotopes / blood Symporters* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 0/Carrier Proteins; 0/Lactates; 0/Rubidium Radioisotopes; 0/Symporters; 0/potassium-chloride symporters; 124-38-9/Carbon Dioxide; 7440-09-7/Potassium; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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