Document Detail

Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter.
MedLine Citation:
PMID:  9435484     Owner:  NLM     Status:  MEDLINE    
K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H(+)-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter, PO2 required for half-maximal activity was 37 +/- 3 mmHg (4.9 kPa). Although RBCs were swollen at low pH, results showed explicitly that the volume change per se had little effect on K influx. Lactate had no effect on volume- or H(+)-stimulated K influxes, nor did bicarbonate or PCO2 affect the magnitude of K influxes after these stimuli or after treatment with protein kinase/phosphatase inhibitors. These results represent the first detailed report of O2 dependence of H(+)-stimulated K-Cl cotransport in RBCs from any mammalian species. They emphasize the importance of PO2 in control of RBC K-Cl cotransport.
P F Speake; C A Roberts; J S Gibson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-09     Completed Date:  1998-02-09     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1811-8     Citation Subset:  IM    
Department of Veterinary Preclinical Sciences, University of Liverpool, United Kingdom.
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MeSH Terms
Bicarbonates / blood
Body Water
Carbon Dioxide / blood
Carrier Proteins / blood*
Erythrocytes / metabolism*
Horses / physiology*
Hydrogen-Ion Concentration
Lactates / blood
Oxygen / blood*
Partial Pressure
Physical Exertion / physiology*
Potassium / blood*
Rubidium Radioisotopes / blood
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Bicarbonates; 0/Carrier Proteins; 0/Lactates; 0/Rubidium Radioisotopes; 0/Symporters; 0/potassium-chloride symporters; 124-38-9/Carbon Dioxide; 7440-09-7/Potassium; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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