Document Detail


Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens.
MedLine Citation:
PMID:  8077407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
Authors:
H D Engler; S T Selepak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  32     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-10-06     Completed Date:  1994-10-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1580-2     Citation Subset:  IM    
Affiliation:
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-0001.
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MeSH Terms
Descriptor/Qualifier:
Centrifugation*
Evaluation Studies as Topic
Humans
Sensitivity and Specificity
Specimen Handling
Time Factors
Virology / instrumentation,  methods*
Virus Diseases / microbiology*
Viruses / isolation & purification*
Comments/Corrections

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