| Effect of cell exposure to top or bottom phase prior to cell partitioning in dextran-poly(ethylene glycol) aqueous phase systems: erythrocytes as a model. | |
| | |
MedLine Citation:
|
PMID: 1375099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cells exposed to dextran (Dx)-rich bottom phase prior to cell partitioning in Dx-poly(ethylene glycol) (PEG) aqueous two-phase systems have lower partition ratios than cells exposed to PEG-rich top phase. Aspects of this previously observed phenomenon were explored. In the present work charge-sensitive phases made with Dx T500 and PEG 8000 were used exclusively. It was found that: (1) even on countercurrent distribution (CCD) red cells (RBC) loaded in bottom phase have a lower apparent partition ratio, G, than the same cells loaded in top phase; (2) when part of the same cell population is loaded into top phase and part into bottom phase of the same load cavities for CCD, with the cells loaded into top or bottom bearing an isotopic tracer (51Cr), the cells loaded into top phase have a higher G value than the cells loaded into bottom phase; (3) the shift in the CCD curves of human or of rat RBC between cells loaded in top or bottom phase using systems having the same polymer concentration (though different salt compositions) shows no striking difference and is, for the number of experiments run, not statistically significant; (4) when the quantity of cells loaded for CCD is reduced from 10(9) to 10(8), the G value of cells loaded in top phase is reduced slightly while that of cells loaded in bottom phase is diminished more appreciably; (5) increasing polymer concentrations yield larger differences in G values between (rat) RBC loaded in top or bottom phase; (6) when cells exposed to top or bottom phase, respectively, are centrifuged and suspended in bottom or top phase, respectively, their CCD patterns are qualitatively similar to cells exposed to these latter respective phases initially; (7) rat RBC populations containing 59Fe-labeled cells of different but distinct age are fractionated on CCD irrespective of whether loaded in top or bottom phase. An exception are populations containing very young mature labeled cells (e.g., 4-d old) which are resolved when loaded in top phase but not in bottom phase. Thus cell populations exist which can be resolved by CCD when loaded in one of the phases but not when loaded in the other. Glutaraldehyde-fixed rat RBC containing 4-d old labeled cells are fractionated by CCD irrespective of whether loaded in top or bottom phase. |
| | |
Authors:
|
H Walter; T J Webber; E J Krob |
Related Documents
:
|
2146239 - Kinetic analysis of 5-fluorouracil action against various cancer cells. 15138059 - Use of a human-derived liver cell line for the detection of cytoprotective, antigenotox... 3908169 - Peripheral t-cell subsets in patients with alopecia areata in different clinical phases. 16668369 - Elicitor-induced ethylene biosynthesis in tomato cells: characterization and use as a b... 1371659 - Variations in rrna content of marine vibrio spp. during starvation-survival and recovery. 15286149 - Physico-chemical characteristics of cell walls from arabidopsis thaliana microcalli sho... 9570939 - An immunohistochemical and morphometric analysis of insulin, insulin-like growth factor... 20639279 - Induction of oxidative stress and cytotoxicity by carbon nanomaterials is dependent on ... 20865129 - The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1105 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1992 Apr |
Date Detail:
|
Created Date: 1992-06-25 Completed Date: 1992-06-25 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 221-9 Citation Subset: IM |
Affiliation:
|
Laboratory of Chemical Biology, Veterans Affairs Medical Center, Long Beach, CA 90822-5201. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Separation* Chromium Countercurrent Distribution Dextrans / pharmacology* Erythrocyte Membrane / drug effects Erythrocytes / drug effects* Humans Male Models, Biological Polyethylene Glycols / pharmacology* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Polyethylene Glycols; 7440-47-3/Chromium; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: [3H]iloprost and prostaglandin E2 compete for the same receptor site on cardiac sarcolemmal membrane...
Next Document: Effects of temperature variation and phenethyl alcohol addition on acyl chain order and lipid organi...