Document Detail


Effect of cell-density on in-vitro dopaminergic differentiation of mesencephalic precursor cells.
MedLine Citation:
PMID:  15770159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural precursor cells isolated from early embryonic mesencephalon are in-vitro expanded and differentiated toward dopamine neurons. However, conditions for controlled conversion of the precursors into dopamine neurons largely remained to be determined. We here examined the effects of plating cell density and duration of in-vitro cell expansion on the precursors-derived dopamine differentiation. The yield of dopamine neurons from cultured mesencephalic precursors was greater when the cells were initially plated at higher density. Soluble factors secreted from the precursors appeared to be responsible for the cell density effect. We further demonstrated that the dopamine differentiation potential of the precursors was lost after a long-term cell expansion. Therefore, in order to attain high percentage of dopamine neuron population in mesencephalic precursor cultures, cultures need to be seeded at high cell density and to be expanded for a short period of time.
Authors:
Ji-Yun Ko; Ji-Yeon Lee; Chang-Hwan Park; Sang-Hun Lee
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  16     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-06-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  499-503     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western / methods
Brain / cytology,  drug effects,  enzymology
Cell Count / methods
Cell Differentiation / drug effects,  physiology*
Cell Proliferation / drug effects
Cells, Cultured
Coculture Techniques / methods
Culture Media, Conditioned / pharmacology
Dopamine / metabolism*
Embryo, Mammalian
Glial Fibrillary Acidic Protein / metabolism
Immunohistochemistry / methods
Mesencephalon / cytology*
Naphthalenes / pharmacology
Neurons / metabolism*
Oxepins / pharmacology
RNA, Messenger / biosynthesis
Rats
Reverse Transcriptase Polymerase Chain Reaction / methods
Stem Cells / physiology*
Time Factors
Tubulin / metabolism
Tyrosine 3-Monooxygenase / metabolism
Chemical
Reg. No./Substance:
0/1-phenyl-1,4-epoxy-1H,4H-naphtho(1,8-de)(1,2)dioxepin; 0/Culture Media, Conditioned; 0/Glial Fibrillary Acidic Protein; 0/Naphthalenes; 0/Oxepins; 0/RNA, Messenger; 0/Tubulin; EC 1.14.16.2/Tyrosine 3-Monooxygenase

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