| Effect of cell cycle inhibitor p19ARF on senescence of human diploid cell. | |
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MedLine Citation:
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PMID: 17447021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the effect of cell cycle inhibitor p14ARF on replicative senescence of human diploid cell, recombinant p19ARF eukaryotic expression vector was constructed and p19ARF gene was transfected into human diploid fibroblasts (WI-38 cells) by liposome-mediated transfection for overexpression. Then, the effects of p19ARF on replicative senescence of WI-38 cells were observed. The results revealed that, compared with control cells, the WI-38 cells in which p19ARF gene was introduced showed significant up-regulation of p53 and p21 expression level, decrease of cell generation by 10-12 generations, decline of cell growth rate with cell cycle being arrested at G1 phase, increase of positive rate of senescent marker SA-beta-gal staining, and decrease of mitochondrial membrane potential. The morphology of the transfected fibroblasts presented the characteristics changes similar to senescent cells. These results indicated that high expression of p19ARF may promote the senescent process of human diploid cells. |
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Authors:
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XueYuan Bai; XiangMei Chen; Kai Hou; Ping Zhang; Zhe Feng; Bo Fu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science in China. Series C, Life sciences / Chinese Academy of Sciences Volume: 50 ISSN: 1006-9305 ISO Abbreviation: Sci. China, C, Life Sci. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-20 Completed Date: 2007-08-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9611809 Medline TA: Sci China C Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 155-60 Citation Subset: IM |
Affiliation:
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Chinese PLA Institute of Nephrology, Chinese PLA General Hospital & Military Medical Postgraduate College, Beijing 100853, China. baixy@301hospital.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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drug effects* Cell Division / drug effects Cell Line Cyclin-Dependent Kinase Inhibitor p16 / pharmacology* Diploidy Flow Cytometry Genes, p53 Genetic Vectors Humans Protein-Serine-Threonine Kinases / genetics Transfection p21-Activated Kinases |
| Chemical | |
Reg. No./Substance:
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0/Cyclin-Dependent Kinase Inhibitor p16; EC 2.7.11.1/PAK1 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/p21-Activated Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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