Document Detail

Effect of cell cycle growth arrest on global DNA methylation status in human lung epithelial-like (A549) cells.
MedLine Citation:
PMID:  17203780     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Decreased global DNA methylation have been previously shown in A549 cells exposed to prolonged hyperoxia. Because hyperoxia induces growth arrest in these cells, whether the status of global DNA methylation changed in response to cellular growth arrest was of interest.
MATERIALS AND METHODS: A549 cells were growth arrested at either the S- or G2/M-phase of the cell cycle by exposure to resveratrol (25 microM), or colcemid (0.1 microg/ml) for 24 h. In addition, to determine the kinetics of hyperoxia-induced growth arrest, cells were exposed to either 1 day of normoxia or 1-5 days of hyperoxia.
RESULTS: The data indicate hyperoxia-induced G2/M growth arrest after day 2 of exposure onwards. Moreover, 66.5% of cells were synchronized at the S-phase after exposure to resveratrol and 97% of them at the G2/M-phase after exposure to colcemid. No changes in global DNA methylation status were observed in cells synchronized at either phase of the cell cycle.
CONCLUSION: These findings indicate that global DNA methylation in A549 cells is not determined primarily by hyperoxia-induced cell cycle growth arrest.
Mihalis I Panayiotidis; Ray C Rancourt; Aglaia Pappa; Carl W White
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  20     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2006 Nov-Dec
Date Detail:
Created Date:  2007-01-05     Completed Date:  2007-02-05     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  861-5     Citation Subset:  IM    
Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology
Cell Cycle / drug effects,  physiology*
Cell Hypoxia
Cell Line, Tumor
Cell Survival / drug effects
DNA / genetics,  metabolism
DNA Methylation*
DNA-Cytosine Methylases / metabolism
Demecolcine / pharmacology
Flow Cytometry
S-Adenosylmethionine / metabolism
Stilbenes / pharmacology
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Stilbenes; 29908-03-0/S-Adenosylmethionine; 477-30-5/Demecolcine; 9007-49-2/DNA; EC 2.1.1.-/DNA modification methylase SssI; EC 2.1.1.-/DNA-Cytosine Methylases; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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