| Effect of celecoxib on cyclooxygenase-1-mediated prostacyclin synthesis and endothelium-dependent contraction in mouse arteries. | |
| | |
MedLine Citation:
|
PMID: 23127498 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This study aimed to determine whether a cyclooxygenase-2 (COX-2) inhibitor celecoxib influences endothelium-dependent contraction independent of its action on COX-2 and, if so, the underlying mechanism(s). Abdominal aortas and/or carotid arteries from C57BL/6 mice or those with genetic COX-2 deficiency (COX-2(-/-)) were isolated for functional and/or biochemical analyses. Result showed that following NO synthase inhibition celecoxib not only reduced the contraction evoked by acetylcholine in C57BL/6 abdominal aorta, but also that in COX-2(-/-) mice showing a comparable magnitude. Notably, the IC(50) of celecoxib obtained in COX-2(-/-) abdominal aorta was only ~0.364μM. Also, celecoxib exhibited a similar effect on COX-2(-/-) carotid arteries. Interestingly, celecoxib was not only found to inhibit the production of the prostacyclin (PGI(2)) metabolite 6-keto-PGF(1α) in COX-2(-/-) aortas, but also caused a reduction in the contraction evoked by PGI(2), by the α(1)-adrenergic agonist phenylephrine, or by 30mM K(+)-induced depolarization in COX-2(-/-) and/or C57BL/6 abdominal aorta. Moreover, N-[2-(cyclohexyloxy)-4-nitrophenyl]-methanesulfonamide (NS398), another COX-2 inhibitor, also reduced the contraction evoked by acetylcholine or by 30mM K(+)-induced depolarization in COX-2(-/-) mice. These results demonstrate explicitly that in mouse arteries celecoxib not only inhibits COX-1-mediated synthesis of PGI(2) and probably some other prostanoids, but also causes a reduction in vessel contractility that is independent of either COX-2 or COX-1, leading to an inhibition of COX-1-mediated endothelium-dependent contraction with an IC(50) value far below that of it considered for COX-1. Also, our data suggest that such effects of celecoxib could be possibly shared by some other COX-2 inhibitors, such as NS398. |
| | |
Authors:
|
Bin Liu; Wenhong Luo; Yingzhan Zhang; Hui Li; Ningxia Zhu; Dongyang Huang; Yingbi Zhou |
Related Documents
:
|
10323128 - Pulmonary edema: a complication following dental treatment under general anesthesia. 2660818 - High-altitude pulmonary edema: a collective review. 1344088 - Radiographic diagnosis of heart failure. 21986438 - Testing the boundaries of neurosurgical innovation … twice: an account of one patient... 18238868 - Endovascular repair of a common iliac artery aneurysm using the cook zenith abdominal a... 10048668 - Post-mortem cast angiography in the diagnostics of graft complications in patients with... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-2 |
Journal Detail:
|
Title: European journal of pharmacology Volume: - ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
|
Cardiovascular Research Center, Shantou University Medical College, Shantou, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-inflammatory effect of berkeleyacetal C through the inhibition of Interleukin-1 receptor-associ...
Next Document: Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates al...