| Effect of cations on the elution of pectic polysaccharides from anion-exchange resins. | |
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MedLine Citation:
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PMID: 8572266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conditions for quantitatively eluting six plant pectic polysaccharides from diethylaminoethyl (DEAE)-columns were established. Cations, surprisingly, greatly affected the elution of pectic polysaccharides from these anion-exchange columns. Quantitative elution of apple pectic acid was achieved when columns of DEAE-Sephadex A-25 and DEAE-Trisacryl Plus-M were developed with 0.5 M NH4Cl, 0.5 M LiCl, or 0.5 M CsCl in buffer. In contrast, up to 1 M NaCl and KCl in buffer only eluted 6.3 to 54% of the apple pectic acid from the two types of columns. In each case the retained apple pectic acid was eluted basically quantitatively by 0.5 M NH4Cl in buffer. The elution of a pectic polysaccharide fraction from Apium graveolens (celery), commercial citrus polygalacturonic acid, and two polysaccharides isolated from purified cell walls of Lemna minor (duckweed) from columns of DEAE-Trisacryl was incomplete when the columns were developed wit 0.5 M NaCl or 0.5 M KCl in buffer, ranging from 0 to 89%; however, again, the retained portion of each sample was eluted quantitatively or almost so from the columns with 0.5 M NH4Cl in buffer. One pectic polysaccharide, commercial citrus pectin, was eluted equally well and almost quantitatively by 0.5 M NaCl, KCl and NH4Cl in buffer. When a pectic polysaccharide fraction from purified cell walls of L. minor was applied to a preparative column of DEAE-Trisacryl Plus-M, 99% of the sample was eluted by a gradient of 0 to 0.5 M NH4Cl in buffer.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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L Cheng; P K Kindel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 228 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1996-03-06 Completed Date: 1996-03-06 Revised Date: 2002-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 109-14 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Michigan State University, East Lansing 48824-1319, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anions Cations Chromatography, Ion Exchange / methods* Plants / chemistry Polysaccharides / isolation & purification* Resins, Plant / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Cations; 0/Polysaccharides; 0/Resins, Plant |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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