| Effect of carvedilol on the production of reactive oxygen species by HL-60 cells. | |
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MedLine Citation:
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PMID: 18987604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The generation of reactive oxygen species (ROS) by phagocytes is one of the irreplaceable microbicidal tools of innate immunity. It has been reported in our previous studies that short-term treatment by carvedilol ex vivo inhibits ROS generation. The purpose of this study was to investigate the long-term effect of carvedilol on phagocytes. METHODS: Human leukemia HL-60 cells differentiated into granulocyte-like cells were used as the model. Final concentrations of carvedilol were 0.1-100 micromol/l. The production of ROS by HL-60 cells was measured using luminol-enhanced chemiluminescence (CL). RESULTS: Carvedilol in concentrations 0.1-10 micromol/l did not exhibit any toxic effect on cells (measured using bioluminescent bacteria Photorhabdus luminescens subsp. thracensis). One hour's treatment with 10 micromol/l carvedilol significantly decreased both spontaneous and activated CL of cells. Conversely, no inhibitory effects on CL were observed in 10 micromol/l carvedilol after 48 h incubation; lower concentrations of carvedilol even slightly increased the CL activity of HL-60 cells. A significant increase in spontaneous CL activity was detected in cells incubated with 10 micromol/l carvedilol in comparison with the control. Powerful antioxidative properties of carvedilol against peroxyl radical (ORAC assay) were proved. No scavenging of nitric oxide (electrochemical method) was observed. CONCLUSIONS: Long-term influence of carvedilol can induce an increase in the generation of phagocyte-derived ROS and potentially also other inflammatory mediators. The increased ROS production is compensated for by antioxidative properties of carvedilol although the increased production of inflammatory mediators could affect the proper function of immune system. |
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Authors:
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Antonin Lojek; Jana Pecivova; Tatiana Macickova; Radomir Nosal; Ivana Papezikova; Milan Ciz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuro endocrinology letters Volume: 29 ISSN: 0172-780X ISO Abbreviation: Neuro Endocrinol. Lett. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-11-25 Completed Date: 2009-02-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008373 Medline TA: Neuro Endocrinol Lett Country: Sweden |
Other Details:
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Languages: eng Pagination: 779-83 Citation Subset: IM |
Affiliation:
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Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic. alojek@ibp.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Adrenergic beta-Antagonists / pharmacology*, toxicity Carbazoles / pharmacology*, toxicity Cell Differentiation / drug effects Cell Proliferation / drug effects Fluorometry Granulocytes / drug effects HL-60 Cells Humans Luminescence Nitric Oxide / metabolism Peroxides / metabolism Phagocytes / drug effects Propanolamines / pharmacology*, toxicity Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic beta-Antagonists; 0/Carbazoles; 0/Peroxides; 0/Propanolamines; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide; 56-65-5/Adenosine Triphosphate; 72956-09-3/carvedilol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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