Document Detail


Effect of carbon nanoparticles on renal epithelial cell structure, barrier function, and protein expression.
MedLine Citation:
PMID:  21067278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess effects of carbon nanoparticle (CNP) exposure on renal epithelial cells, fullerenes (C(60)), single-walled carbon nanotubes (SWNT), and multi-walled carbon nanotubes (MWNT) were incubated with a confluent renal epithelial line for 48 h. At low concentrations, CNP-treated cells exhibited significant decreases in transepithelial electrical resistance (TEER) but no changes in hormone-stimulated ion transport or CNP-induced toxicity or stress responses as measured by lactate dehydrogenase or cytokine release. The changes in TEER, manifested as an inverse relationship with CNP concentration, were mirrored by an inverse correlation between dose and changes in protein expression. Lower, more physiologically relevant, concentrations of CNP have the most profound effects on barrier cell function and protein expression. These results indicate an impact of CNPs on renal epithelial cells at concentrations lower than have been previously studied and suggest caution with regard to increasing CNP levels entering the food chain due to increasing environmental pollution.
Authors:
Bonnie L Blazer-Yost; Amiraj Banga; Adam Amos; Ellen Chernoff; Xianyin Lai; Cheng Li; Somenath Mitra; Frank A Witzmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-10
Journal Detail:
Title:  Nanotoxicology     Volume:  5     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-25     Completed Date:  2011-12-29     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  England    
Other Details:
Languages:  eng     Pagination:  354-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon / chemistry,  toxicity*
Cell Line
Epithelial Cells / cytology,  drug effects*
Fullerenes
Kidney Tubules, Collecting / cytology*
Mice
Microscopy, Atomic Force
Nanoparticles / chemistry,  toxicity*,  ultrastructure
Time Factors
X-Ray Therapy
Grant Support
ID/Acronym/Agency:
R01 GM085218/GM/NIGMS NIH HHS; R01 GM085218-04/GM/NIGMS NIH HHS; R01 GM085218-05/GM/NIGMS NIH HHS; R01GM085218/GM/NIGMS NIH HHS; RC2 ES018810-02/ES/NIEHS NIH HHS; RC2ES018810/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Fullerenes; 7440-44-0/Carbon
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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