| Effect of captopril and the bradykinin-PKC pathway on ROS production in type 1 diabetic rats. | |
| | |
MedLine Citation:
|
PMID: 22117100 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to investigate the possible effects of captopril as a promoter in modulating the oxidant-antioxidant balance in rats with type 1 diabetes, and the influence of protein kinase C (PKC) pathways in the production of reactive oxygen species (ROS) induced by bradykinin in type 1 diabetic rats. This study evaluated the redox status in both the cardiac tissue and at the cellular level (neutrophils). Two concentrations of captopril were utilized: (i) 5 mg·(kg body mass)(-1), which was considered a therapeutic dose; and (ii) 10 mg·(kg body mass)(-1). Body mass, plasma glucose, and serum insulin were evaluated. To investigate the redox status of the cardiac tissue, we analyzed lipid peroxidation, concentration of carbonylated protein, catalase activity, and the concentration of glutathione. For a more accurate assessment of the possible antioxidant effect of captopril, we also analyzed ROS in neutrophils (in vivo), and ROS production induced by bradykinin and the influence of the PKC pathway in this production (in vitro). Our data show that the hearts of diabetic animals have increased oxidative damage, exemplified by the increased concentration of carbonylated protein and thiobarbituric acid reactive substances (TBARS). However, animals treated with captopril at both concentrations showed lower concentrations of carbonylated protein compared with untreated diabetic animals. We found an increase of catalase activity in the heart of diabetic rats, which was reversed by captopril treatment at both of the dosages tested. Our data showed that captopril was able to reduce ROS production in the neutrophils of diabetic rats at a dose of 10 mg captopril·(kg body mass)(-1). However, the antioxidant effect of captopril is independent of bradykinin. Diabetes induces oxidative stress, and these results suggest that captopril has an antioxidant effect and can modulate the production of ROS in circulating neutrophils. |
| | |
Authors:
|
Glaucy Rodrigues de Araujo; Karine Granato de Faria; Wanderson Geraldo Lima; Bruno da Cruz Pádua; Joamyr Victor Rossoni; Aline Arlindo Souza; Deoclecio Chianca-Júnior; Marcelo Eustáquio Silva; Maria Lucia Pedrosa; Miriam Martins Chaves; Daniela Caldeira Costa |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-25 |
Journal Detail:
|
Title: Canadian journal of physiology and pharmacology Volume: - ISSN: 1205-7541 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
a Programa de Pós-graduação em Ciências Biológicas do Núcleo de Pesquisas em Ciências Biológicas - NUPEB, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, 35400-000, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spotlight on rotigotine transdermal patch in Parkinson's disease†.
Next Document: Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise...