Document Detail

Effect of capric, lauric and alpha-linolenic acids on the division time distributions of single cells of Staphylococcus aureus.
MedLine Citation:
PMID:  18793815     Owner:  NLM     Status:  MEDLINE    
The effect of non-inhibitory concentrations of capric, lauric and alpha-linolenic acids (C10:0, C12:0 and C18:3 respectively) on the division time distribution of single cells of Staphylococcus aureus was evaluated at pH 7 and pH 5. The effect of the initial cell concentration on the lag time of growing cell populations was also assessed. The statistical properties of the division times (defined as the time interval from birth to next binary fission for a single cell) were studied using the method of Elfwing et al. [Elfwing, A., Le Marc, Y., Baranyi, J., Ballagi, A., 2004. Observing the growth and division of large number of individual bacteria using image analysis. Applied and Environmental Microbiology 70, 675-678]. The division times were significantly longer in the presence of free fatty acids than in the control. Shorter division intervals were detected at pH 7 than at pH 5 in the control experiment and in the presence of C10:0. However, both C12:0 and C18:3 slowed down the growth, regardless of the pH. The observed division time distributions were used to simulate growth curves from different inoculum sizes using the stochastic birth process described by Pin and Baranyi [Pin, C., Baranyi, J., 2006. Kinetics of single cells: observation and modelling of a stochastic process. Applied and Environmental Microbiology 72, 2163-2169]. The output of the simulation results were compared with observed data. The lag times fitted to simulated growth curves were in good agreement with those fitted to growth curves measured by plate counts. The averaged out effect of the population masked the effect of the free fatty acids and pH on the division times of single cells.
S Sado Kamdem; M E Guerzoni; J Baranyi; C Pin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-09
Journal Detail:
Title:  International journal of food microbiology     Volume:  128     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2009-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  122-8     Citation Subset:  IM    
Dipartimento di Scienze degli Alimenti (DISA), University of Bologna, Campus Scienze degli Alimenti, Pzza Goidanich, 60, 47023 Cesena, Italy.
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MeSH Terms
Cell Division / physiology*
Colony Count, Microbial
Decanoic Acids / pharmacology*
Food Microbiology
Hydrogen-Ion Concentration
Lauric Acids / pharmacology*
Models, Biological*
Population Density
Predictive Value of Tests
Staphylococcus aureus / cytology,  drug effects,  growth & development*
Stochastic Processes
Time Factors
alpha-Linolenic Acid / pharmacology*
Reg. No./Substance:
0/Decanoic Acids; 0/Lauric Acids; 143-07-7/lauric acid; 334-48-5/decanoic acid; 463-40-1/alpha-Linolenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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