Document Detail

Effect of caffeine on radiation-induced apoptosis in TK6 cells.
MedLine Citation:
PMID:  7838955     Owner:  NLM     Status:  MEDLINE    
Apoptosis has been measured in cells of the human TK6 lymphoblastoid cell line by recording the release of endonuclease-digested DNA from affected cells using flow cytometry. In asynchronously dividing cells, DNA degradation characteristic of apoptosis was first seen 12 h after irradiation as a defined DNA fluorescent peak of sub-G1-phase content, reaching a maximum of 30-50% of the population by 24-72 h. Treating cells with 2 mM caffeine either before or up to 3 h after irradiation eliminated the degradation of DNA entirely. In addition, the percentage of cells in which apoptosis could be detected microscopically decreased from 62.4 +/- 0.95% to 16.7 +/- 1.5% 72 h after caffeine treatment. Delaying caffeine treatment for 12 h after irradiation reduced DNA degradation by approximately 50% compared to cells receiving radiation alone. DNA degradation induced by serum deprivation was unaffected by caffeine treatment. These data support the contention that irradiation of TK6 cells produces a long-lived cellular signal which triggers apoptosis. Apoptosis produced by serum deprivation does not operate through the same pathway.
W Zhen; A T Vaughan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  141     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-02-28     Completed Date:  1995-02-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  170-5     Citation Subset:  IM; S    
Department of Radiotherapy, Loyola University Chicago, Maywood, Illinois 60153.
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MeSH Terms
Apoptosis / drug effects*,  radiation effects*
Caffeine / pharmacology*
Cell Line
Culture Media
DNA / drug effects
Dose-Response Relationship, Radiation
G2 Phase / drug effects,  radiation effects
Grant Support
Reg. No./Substance:
0/Culture Media; 58-08-2/Caffeine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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