| Effect of caffeine on radiation-induced apoptosis in TK6 cells. | |
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MedLine Citation:
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PMID: 7838955 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis has been measured in cells of the human TK6 lymphoblastoid cell line by recording the release of endonuclease-digested DNA from affected cells using flow cytometry. In asynchronously dividing cells, DNA degradation characteristic of apoptosis was first seen 12 h after irradiation as a defined DNA fluorescent peak of sub-G1-phase content, reaching a maximum of 30-50% of the population by 24-72 h. Treating cells with 2 mM caffeine either before or up to 3 h after irradiation eliminated the degradation of DNA entirely. In addition, the percentage of cells in which apoptosis could be detected microscopically decreased from 62.4 +/- 0.95% to 16.7 +/- 1.5% 72 h after caffeine treatment. Delaying caffeine treatment for 12 h after irradiation reduced DNA degradation by approximately 50% compared to cells receiving radiation alone. DNA degradation induced by serum deprivation was unaffected by caffeine treatment. These data support the contention that irradiation of TK6 cells produces a long-lived cellular signal which triggers apoptosis. Apoptosis produced by serum deprivation does not operate through the same pathway. |
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Authors:
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W Zhen; A T Vaughan |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Radiation research Volume: 141 ISSN: 0033-7587 ISO Abbreviation: Radiat. Res. Publication Date: 1995 Feb |
Date Detail:
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Created Date: 1995-02-28 Completed Date: 1995-02-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0401245 Medline TA: Radiat Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 170-5 Citation Subset: IM; S |
Affiliation:
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Department of Radiotherapy, Loyola University Chicago, Maywood, Illinois 60153. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects*,
radiation effects* Blood Caffeine / pharmacology* Cell Line Culture Media DNA / drug effects Dose-Response Relationship, Radiation G2 Phase / drug effects, radiation effects Humans |
| Grant Support | |
ID/Acronym/Agency:
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CA55840/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 58-08-2/Caffeine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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