Document Detail


Effect of caffeine on mucus secretion and agonist-dependent Ca2+ mobilization in human gastric mucus secreting cells.
MedLine Citation:
PMID:  9150277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caffeine is known to stimulate gastric acid secretion, but, the effects of caffeine on gastric mucus secretion have not been clarified. To elucidate the action of caffeine on gastric mucin-producing cells and its underlying mechanism, the effects of caffeine on mucus glycoprotein secretion and agonist-induced [Ca2+]i mobilization were examined in human gastric mucin secreting cells (JR-I cells). The measurement of [Ca2+]i using Indo-1 and the whole cell voltage clamp technique were applied. Mucus glycoprotein secretion was assessed by release of [3H]glucosamine. Caffeine by itself failed to increase [Ca2+]i and affect membrane currents, while it dose-dependently inhibited agonist (acetylcholine (ACh) or histamine)-induced [Ca2+]i rise, resulting in inhibiting activation of Ca2+-dependent K+ current (I(K.Ca)) evoked by agonists. The effect of caffeine was reversible, and the half maximal inhibitory concentration was about 0.5 mM. But, caffeine did not suppress [Ca2+]i rise and activation of I(K.Ca) induced by A23187 or inositol trisphosphate (IP3). Theophylline or 3-isobutyl-1-methyl-xanthine (IBMX) did not mimic the effect of caffeine. Caffeine failed to stimulate mucus secretion, while it significantly decreased ACh-induced mucus secretion. These results indicate that caffeine selectively inhibits agonist-mediated [Ca2+]i rise in human gastric epithelial cells, probably through the blockade of receptor-IP3 signaling pathway, which may affect the mucin secretion.
Authors:
E Hamada; T Nakajima; Y Hata; H Hazama; K Iwasawa; M Takahashi; S Ota; M Omata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1356     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-28     Completed Date:  1997-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  198-206     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
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MeSH Terms
Descriptor/Qualifier:
Caffeine / pharmacology*
Calcimycin
Calcium / metabolism*
Gastric Mucosa / drug effects*,  metabolism
Humans
Indoles
Inositol Phosphates
Mucins / analysis
Mucus / chemistry,  secretion*
Spectrometry, Fluorescence
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Indoles; 0/Inositol Phosphates; 0/Mucins; 52665-69-7/Calcimycin; 58-08-2/Caffeine; 7440-70-2/Calcium; 96314-96-4/indo-1

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