Document Detail

Effect of caffeine on mucus secretion and agonist-dependent Ca2+ mobilization in human gastric mucus secreting cells.
MedLine Citation:
PMID:  9150277     Owner:  NLM     Status:  MEDLINE    
Caffeine is known to stimulate gastric acid secretion, but, the effects of caffeine on gastric mucus secretion have not been clarified. To elucidate the action of caffeine on gastric mucin-producing cells and its underlying mechanism, the effects of caffeine on mucus glycoprotein secretion and agonist-induced [Ca2+]i mobilization were examined in human gastric mucin secreting cells (JR-I cells). The measurement of [Ca2+]i using Indo-1 and the whole cell voltage clamp technique were applied. Mucus glycoprotein secretion was assessed by release of [3H]glucosamine. Caffeine by itself failed to increase [Ca2+]i and affect membrane currents, while it dose-dependently inhibited agonist (acetylcholine (ACh) or histamine)-induced [Ca2+]i rise, resulting in inhibiting activation of Ca2+-dependent K+ current (I(K.Ca)) evoked by agonists. The effect of caffeine was reversible, and the half maximal inhibitory concentration was about 0.5 mM. But, caffeine did not suppress [Ca2+]i rise and activation of I(K.Ca) induced by A23187 or inositol trisphosphate (IP3). Theophylline or 3-isobutyl-1-methyl-xanthine (IBMX) did not mimic the effect of caffeine. Caffeine failed to stimulate mucus secretion, while it significantly decreased ACh-induced mucus secretion. These results indicate that caffeine selectively inhibits agonist-mediated [Ca2+]i rise in human gastric epithelial cells, probably through the blockade of receptor-IP3 signaling pathway, which may affect the mucin secretion.
E Hamada; T Nakajima; Y Hata; H Hazama; K Iwasawa; M Takahashi; S Ota; M Omata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1356     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-28     Completed Date:  1997-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  198-206     Citation Subset:  IM    
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
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MeSH Terms
Caffeine / pharmacology*
Calcium / metabolism*
Gastric Mucosa / drug effects*,  metabolism
Inositol Phosphates
Mucins / analysis
Mucus / chemistry,  secretion*
Spectrometry, Fluorescence
Tumor Cells, Cultured
Reg. No./Substance:
0/Indoles; 0/Inositol Phosphates; 0/Mucins; 52665-69-7/Calcimycin; 58-08-2/Caffeine; 7440-70-2/Calcium; 96314-96-4/indo-1

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