Document Detail

Effect of cadmium on the relationship between replicative and repair DNA synthesis in synchronized CHO cells.
MedLine Citation:
PMID:  11054109     Owner:  NLM     Status:  MEDLINE    
Repair and replicative DNA synthesis were measured at different stages of the cell cycle in control and cadmium-treated Chinese hamster ovary (CHO-K1) cells. Cells were synchronized by counterflow centrifugal elutriation. Elutriation resulted in five repair and four replication subphases. On Cd treatment, repair synthesis was elevated in certain subphases. Replicative subphases were suppressed by Cd treatment, with some of the peaks almost invisible. The number of spontaneous strand breaks measured by random oligonucleotide primed synthesis assay showed a cell-cycle-dependent fluctuation in control cells and was greatly increased after Cd treatment throughout the S phase. Elevated levels of the oxidative DNA damage product, 8-oxodeoxyguanosine, were observed after Cd treatment, with the highest level in early S phase, which gradually declined as damaged cells progressed through the cell cycle.
G Banfalvi; N Littlefield; B Hass; M Mikhailova; I Csuka; E Szepessy; M W Chou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  267     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-05     Completed Date:  2000-12-28     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  6580-5     Citation Subset:  IM    
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University Medical School, Budapest, Hungary.
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MeSH Terms
Adenosine Triphosphate / metabolism
CHO Cells
Cadmium Chloride / pharmacology*
Cell Division
DNA / chemistry,  genetics
DNA Primers
DNA Repair / drug effects*
DNA Replication / drug effects*
Deoxyguanosine / analogs & derivatives*,  analysis
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/DNA Primers; 10108-64-2/Cadmium Chloride; 56-65-5/Adenosine Triphosphate; 9007-49-2/DNA; 961-07-9/Deoxyguanosine

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