Document Detail


Effect of c-myc on the ultrastructural structure of cochleae in guinea pigs with noise induced hearing loss.
MedLine Citation:
PMID:  19785991     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Noise over-stimulation may induce hair cells loss and hearing deficit. The c-myc oncogene is a major regulator for cell proliferation, growth, and apoptosis. However, the role of this gene in the mammalian cochlea is still unclear. The study was designed to firstly investigate its function under noise condition, from the aspect of cochlear ultrastructural changes. We had established the adenoviral vector of c-myc gene and delivered the adenovirus suspension into the scala tympani of guinea pigs 4 days before noise exposure. The empty adenoviral vectors were injected as control. Then, all subjects were exposed to 4-kHz octave-band noise at 110dB SPL for 8h/day, 3 days consecutively. Auditory thresholds were assessed by auditory brainstem response, prior to and 7 days following noise exposure. On the seventh days after noise exposure, the cochlear sensory epithelia surface was observed microscopically and the cochleae were taken to study the ultrastructural changes. The results indicated that auditory threshold shift after noise exposure was higher in the ears treated with Ad.EGFP than that treated with Ad.c-myc-EGFP. Stereocilia loss and the disarrangement of outer hair cells were observed, with greater changes found in the Ad.EGFP group. Also, the ultrastructure changes were severe in the Ad.EGFP group, but not obvious in the Ad.c-myc-EGFP group. Therefore, c-myc gene might play an unexpected role in hearing functional and morphological protection from acoustic trauma.
Authors:
Yu Han; Cuiping Zhong; Liu Hong; Ye Wang; Li Qiao; Jianhua Qiu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-26
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  390     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2009-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  458-62     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032 Shaanxi Province, China.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae
Animals
Cochlea / metabolism,  ultrastructure*
Guinea Pigs
Hearing Loss / etiology*,  metabolism,  pathology*
Noise / adverse effects*
Proto-Oncogene Proteins c-myc / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-myc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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