Document Detail

Effect of butyrate on glycolipid metabolism of two cell types of rat ascites hepatomas with different ganglioside biosynthesis.
MedLine Citation:
PMID:  521438     Owner:  NLM     Status:  MEDLINE    
The effect of butyrate on glycolipid metabolism and morphological differentiation in the cell culture system of rat ascites hepatomas, AH 7974 of island-forming type and AH 7974F of free type, was studied. Both cell lines adhered to the substratum in the presence of 1 mM butyrate. In the case of AH 7974, the addition of butyrate induced a distinct morphological change but the other cell line showed no such conspicuous change. Butyrate-treated AH 7974 cells showed a 2 to 3-fold elevation of CMP-N-acetylneuraminic acid: lactosylceramide sialyltransferase activity to form N-acetylneuraminylgalactosylglucosylceramide (GM3). On the other hand, no enzyme activity could be detected in AH 7974F cells. Four glycosyltransferase activities involved in glycolipid synthesis, including sialyltransferase in AH 7974F cells, were reduced by butyrate. From these observations we concluded that sialyltransferase to form GM3, or TM3 itself, is prerequisite for the morphological alteration induced by butyrate.
T Taki; Y Hirabayashi; R Kondo; M Matsumoto; K Kojima
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  86     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-03-17     Completed Date:  1980-03-17     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1395-402     Citation Subset:  IM    
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MeSH Terms
Butyrates / pharmacology*
Cell Division / drug effects
Cell Line
Cytidine Monophosphate N-Acetylneuraminic Acid / metabolism
Galactosyltransferases / metabolism
Gangliosides / biosynthesis*
Glucosamine / metabolism
Glycolipids / metabolism*
Liver Neoplasms, Experimental / metabolism*
Transferases / metabolism
Reg. No./Substance:
0/Butyrates; 0/Gangliosides; 0/Glycolipids; 3063-71-6/Cytidine Monophosphate N-Acetylneuraminic Acid; 3416-24-8/Glucosamine; EC 2.-/Transferases; EC 2.4.1.-/Galactosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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