Document Detail

Effect of branched chain alpha-keto acids on kynurenine 3-hydroxylase from rat liver.
MedLine Citation:
PMID:  7131097     Owner:  NLM     Status:  MEDLINE    
In the preceding paper, we found that branched chain alpha-keto acids, alpha-ketoglutarate and pyruvate were inhibitory to kynurenine 3-hydroxylase [EC] from Saccharomyces (Shin, M. et al. (1982): J. Nutr. Sci. vitaminol., 28, 191-201). As kynurenine 3-hydroxylase is reported to be a rate-limiting enzyme in the tryptophan-NAD pathway in rats, branched chain amino acids, branched chain alpha-keto acids and several other keto acids were tested for their effects on kynurenine 3-hydroxylase activity in the mitochondrial outer membrane fraction prepared from rat liver. In contrast with the yeast enzyme, the rat liver enzyme was resistant to alpha-keto acid-inhibition and more than 70% of the enzyme activity was maintained even in the presence of 20 mM of each alpha-keto acid. The present result implies that the mechanism of the hepatic NAD-lowering effect of leucine in rats might be completely different from that operative in Saccharomyces.
M Shin; O Yamada; K Sano; C Umezawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of nutritional science and vitaminology     Volume:  28     ISSN:  0301-4800     ISO Abbreviation:  J. Nutr. Sci. Vitaminol.     Publication Date:  1982  
Date Detail:
Created Date:  1982-12-21     Completed Date:  1982-12-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0402640     Medline TA:  J Nutr Sci Vitaminol (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  203-7     Citation Subset:  IM    
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MeSH Terms
Amino Acids, Branched-Chain / pharmacology*
Intracellular Membranes / enzymology
Keto Acids / pharmacology*
Kynurenine 3-Monooxygenase
Mitochondria, Liver / enzymology*
Mixed Function Oxygenases / antagonists & inhibitors*
Rats, Inbred Strains
Structure-Activity Relationship
Subcellular Fractions / enzymology
Reg. No./Substance:
0/Amino Acids, Branched-Chain; 0/Keto Acids; EC 1.-/Mixed Function Oxygenases; EC 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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