| Effect of bottom clearance on performance of airlift bioreactor in high-density culture of Panax notoginseng cells. | |
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MedLine Citation:
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PMID: 16233116 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A fed-batch cultivation of Panax notoginseng cells in a concentric-tube airlift reactor was performed to study the effects of bottom clearance on cell growth and the production of ginseng saponin and polysaccharide. At a bottom clearance of 4.0 cm, the highest cell density of 29.1+/-1.6 g/l by dry weight was obtained, and the accumulation of saponin and polysaccharide also reached a maximum, i.e., 2.39+/-0.43 and 2.73+/-0.40 g/l, respectively. Cell growth and metabolite production were limited at a small (2.5 cm) or large (5.0 cm) bottom clearance. By analyzing the time constants of mixing, mass transfer and oxygen consumption, bulk gas-liquid oxygen transfer was found to be responsible for the growth limitation at a small bottom clearance (2.5 cm). The decrease in cell density at a large bottom clearance (5.0 cm) was related to cell sedimentation at the reactor bottom. This work is beneficial for the scale-up and efficient operation of the airlift reactor in cell cultures. |
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Authors:
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W Hu; J Zhong |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 92 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 389-92 Citation Subset: - |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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