Document Detail

Effect of bone marrow-derived extracellular matrix on cardiac function after ischemic injury.
MedLine Citation:
PMID:  22819498     Owner:  NLM     Status:  MEDLINE    
Ischemic heart disease is a leading cause of death, with few options to retain ventricular function following myocardial infarction. Hematopoietic-derived progenitor cells contribute to angiogenesis and tissue repair following ischemia reperfusion injury. Motivated by the role of bone marrow extracellular matrix (BM-ECM) in supporting the proliferation and regulation of these cell populations, we investigated BM-ECM injection in myocardial repair. In BM-ECM isolated from porcine sternum, we identified several factors important for myocardial healing, including vascular endothelial growth factor, basic fibroblast growth factor-2, and platelet-derived growth factor-BB. We further determined that BM-ECM serves as an adhesive substrate for endothelial cell proliferation. Bone marrow ECM was injected in a rat model of myocardial infarction, with and without a methylcellulose carrier gel. After one day, reduced infarct area was noted in rats receiving BM-ECM injection. After seven days we observed improved fractional shortening, decreased apoptosis, and significantly lower macrophage counts in the infarct border. Improvements in fractional shortening, sustained through 21 days, as well as decreased fibrotic area, enhanced angiogenesis, and greater c-kit-positive cell presence were associated with BM-ECM injection. Notably, the concentrations of BM-ECM growth factors were 10(3)-10(8) fold lower than typically required to achieve a beneficial effect, as reported in pre-clinical studies that have administered single growth factors alone.
Swathi Ravi; Jeffrey M Caves; Adam W Martinez; Jiantao Xiao; Jing Wen; Carolyn A Haller; Michael E Davis; Elliot L Chaikof
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-21
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2012-12-26     Revised Date:  2013-12-10    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  7736-45     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Bone Marrow / metabolism*
Bone Marrow Cells / cytology
Cell Adhesion
Cell Proliferation
Extracellular Matrix / metabolism*
Heart Function Tests*
Human Umbilical Vein Endothelial Cells
Methylcellulose / chemistry
Myocardial Ischemia / physiopathology*,  therapy
Rats, Sprague-Dawley
Stem Cell Transplantation
Sus scrofa
Grant Support
Reg. No./Substance:

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