| Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718. | |
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MedLine Citation:
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PMID: 20506102 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aim of the present study was to assess and evaluate the impact of various kinds of impurities of biodiesel-derived raw glycerol feedstock, upon microbial growth and 1,3-propanediol (1,3-PDO) production by Clostridium butyricum. Preliminary trials in 200-mL anaerobic bottles revealed that the presence of NaCl at a concentration of 4.5% (w/w of glycerol) in growth medium imposed an evident inhibitory effect, in contrast with phosphoric salts. However, the application of NaCl at elevated quantities during batch bioreactor experiments [up to 30% (w/w of glycerol)], did neither affect the microbial growth, nor the 1,3-PDO production. Moreover, when oleic acid was added into the growth medium at 2% (w/w of glycerol), a total preclusion of the strain was observed. In order to further investigate whether the nature of oleic acid itself or the presence of the double bond induced the inhibitory phenomenon, stearic acid was added into the medium at the same concentration (2%, w/w, of glycerol). Indeed, no inhibitory effect was observed in the fermentor, suggesting that the presence of the double bond may play a key role in the growth behavior of the microorganism. Finally, methanol effect was tested in batch and continuous bioreactor operations. Interestingly enough, the alcohol addition did not affect the microbial bioconversion of glycerol into 1,3-PDO, even when imposed at relatively high concentrations (10%, w/w, of glycerol) in batch-bioreactor operations. In continuous experiments, methanol was added when steady state had been achieved, and although in one case high concentration was added into the chemostat (5 g/L), the system re-obtained a steady state without indications of negative effect upon biomass production due to the alcohol. |
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Authors:
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Afroditi Chatzifragkou; David Dietz; Michael Komaitis; An-Ping Zeng; Seraphim Papanikolaou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 107 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-11-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 76-84 Citation Subset: IM |
Copyright Information:
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2010 Wiley Periodicals, Inc. |
Affiliation:
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Department of Food Science and Technology, Agricultural University of Athens, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biofuels
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microbiology* Cell Proliferation / drug effects Clostridium butyricum / drug effects, physiology* Dose-Response Relationship, Drug Glycerol / administration & dosage* Industrial Waste / prevention & control Propylene Glycols / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Biofuels; 0/Industrial Waste; 0/Propylene Glycols; 504-63-2/1,3-propanediol; 56-81-5/Glycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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