Document Detail


Effect of biliary obstruction on 26-hydroxylation of C27-steroids in bile acid synthesis.
MedLine Citation:
PMID:  632686     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of biliary obstruction on side chain hydroxylations in the biosynthesis and metabolism of bile acids was studied in the rat. For comparison, several other hydroxylation reactions in bile acid biosynthesis and metabolism were assayed. Biliary obstruction inhibited microsomal 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol and microsomal 25- and 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-12alpha-triol. Microsomal 7alpha-hydroxylation of cholesterol and 6beta-hydroxylation of lithocholic acid acid increased significantly, whereas the increase in microsomal 12alpha-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol was less. Mitochondrial 26-hydroxylation of cholesterol, 5-cholestene-3beta,7alpha-diol, and 7alpha-hydroxy-4-cholesten-3-one was stimulated, whereas 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol was not affected and that of 5beta-cholestane-3alpha,7alpha,12alpha-triol was markedly inhibited. The results indicate that mitochondrial 26-hydroxylation, particularly of substrates that primarily are precursors of chenodeoxycholic acid, plays a more important role in bile acid biosynthesis under conditions of biliary obstruction than under normal conditions.
Authors:
J Gustafsson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  19     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-05-08     Completed Date:  1978-05-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / biosynthesis*
Bile Ducts / physiology*
Kinetics
Ligation
Liver / metabolism*
Male
Microsomes, Liver / metabolism
Mitochondria, Liver / metabolism
Rats
Steroid Hydroxylases / metabolism*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; EC 1.14.-/Steroid Hydroxylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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