Document Detail

Effect of biliary obstruction on 26-hydroxylation of C27-steroids in bile acid synthesis.
MedLine Citation:
PMID:  632686     Owner:  NLM     Status:  MEDLINE    
The effect of biliary obstruction on side chain hydroxylations in the biosynthesis and metabolism of bile acids was studied in the rat. For comparison, several other hydroxylation reactions in bile acid biosynthesis and metabolism were assayed. Biliary obstruction inhibited microsomal 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol and microsomal 25- and 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-12alpha-triol. Microsomal 7alpha-hydroxylation of cholesterol and 6beta-hydroxylation of lithocholic acid acid increased significantly, whereas the increase in microsomal 12alpha-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol was less. Mitochondrial 26-hydroxylation of cholesterol, 5-cholestene-3beta,7alpha-diol, and 7alpha-hydroxy-4-cholesten-3-one was stimulated, whereas 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol was not affected and that of 5beta-cholestane-3alpha,7alpha,12alpha-triol was markedly inhibited. The results indicate that mitochondrial 26-hydroxylation, particularly of substrates that primarily are precursors of chenodeoxycholic acid, plays a more important role in bile acid biosynthesis under conditions of biliary obstruction than under normal conditions.
J Gustafsson
Related Documents :
7448336 - Biliary excretion of chlorthalidone in humans.
10923826 - Studies on adsorption characteristics of bile acids and methotrexate to a new type of a...
2703966 - Role of bile acid conjugation in hepatic transport of dihydroxy bile acids.
744496 - Kinetics of 14c-glycocholic acid clearance in normal man and in patients with liver dis...
23131896 - Suppression of ccr impacts metabolite profile and cell wall composition in pinus radiat...
17060916 - Fret analysis of protein conformational change through position-specific incorporation ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  19     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-05-08     Completed Date:  1978-05-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-43     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bile Acids and Salts / biosynthesis*
Bile Ducts / physiology*
Liver / metabolism*
Microsomes, Liver / metabolism
Mitochondria, Liver / metabolism
Steroid Hydroxylases / metabolism*
Reg. No./Substance:
0/Bile Acids and Salts; EC 1.14.-/Steroid Hydroxylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determinants of fatty acid and alcohol monomer activities in mixed micellar solutions.
Next Document:  Ruminal hydrogenation of cholesterol.