Document Detail


Effect of bile duct ligation on bile acid composition in mouse serum and liver.
MedLine Citation:
PMID:  22098667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cholestatic liver diseases can be caused by genetic defects, drug toxicities, hepatobiliary malignancies or obstruction of the biliary tract. Cholestasis leads to accumulation of bile acids (BAs) in hepatocytes. Direct toxicity of BAs is currently the most accepted hypothesis for cholestatic liver injury. However, information on which bile acids are actually accumulating during cholestasis is limited.
AIM: To assess the BA composition in liver and serum after bile duct ligation (BDL) in male C57Bl/6 mice between 6 h and 14 days and evaluate toxicity of the most abundant BAs.
RESULTS: Bile acid concentrations increased in liver (27-fold) and serum (1400-fold) within 6 h after surgery and remained elevated up to 14 days. BAs in livers of BDL mice became more hydrophilic than sham controls, mainly because of increased 6β-hydroxylation and taurine conjugation. Among the eight unconjugated and 16 conjugated BAs identified in serum and liver, only taurocholic acid (TCA), β-muricholic acid (βMCA) and TβMCA were substantially elevated representing >95% of these BAs over the entire time course. Although glycochenodeoxycholic acid and other conjugated BAs increased in BDL animals, the changes were several orders of magnitude lower compared with TCA, βMCA and TβMCA. A mixture of these BAs did not cause apoptosis or necrosis, but induced inflammatory gene expression in cultured murine hepatocytes.
CONCLUSION: The concentrations of cytotoxic BAs are insufficient to cause hepatocellular injury. In contrast, TCA, βMCA and TβMCA are able to induce pro-inflammatory mediators in hepatocytes. Thus, BAs act as inflammagens and not as cytotoxic mediators after BDL in mice.
Authors:
Youcai Zhang; Ji-Young Hong; Cheryl E Rockwell; Bryan L Copple; Hartmut Jaeschke; Curtis D Klaassen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-17
Journal Detail:
Title:  Liver international : official journal of the International Association for the Study of the Liver     Volume:  32     ISSN:  1478-3231     ISO Abbreviation:  Liver Int.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-14     Completed Date:  2012-04-19     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  101160857     Medline TA:  Liver Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  58-69     Citation Subset:  IM    
Copyright Information:
© 2011 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / chemistry,  metabolism*,  pharmacology
Bile Ducts, Extrahepatic / surgery
Cell Adhesion Molecules / genetics,  metabolism
Cholestasis, Extrahepatic / blood*,  etiology,  pathology
Cytokines / genetics,  metabolism
Disease Models, Animal
Gene Expression
Hepatocytes / drug effects,  metabolism,  pathology
Inflammation Mediators / metabolism
Ligation
Liver / metabolism*,  pathology
Male
Mice
Mice, Inbred C57BL
RNA, Messenger / metabolism
Grant Support
ID/Acronym/Agency:
AA12916/AA/NIAAA NIH HHS; DK073566/DK/NIDDK NIH HHS; ES-009649/ES/NIEHS NIH HHS; ES-009716/ES/NIEHS NIH HHS; ES-013714/ES/NIEHS NIH HHS; P20 RR021940/RR/NCRR NIH HHS; P20 RR021940/RR/NCRR NIH HHS; P20 RR021940-07/RR/NCRR NIH HHS; R01 ES009649/ES/NIEHS NIH HHS; R01 ES009649-10/ES/NIEHS NIH HHS; R01 ES009716/ES/NIEHS NIH HHS; R01 ES009716-10/ES/NIEHS NIH HHS; R01 ES013714/ES/NIEHS NIH HHS; R01 ES013714-05/ES/NIEHS NIH HHS; RR-021940/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cell Adhesion Molecules; 0/Cytokines; 0/Inflammation Mediators; 0/RNA, Messenger
Comments/Corrections

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