Document Detail

Effect of bile acid on the cell membrane functionality of lactic acid bacteria for oral administration.
MedLine Citation:
PMID:  16730163     Owner:  NLM     Status:  MEDLINE    
Lactic acid bacteria and other species dwelling in the gut must be tolerant to bile salts. This study sought to determine the effects of the bile salts taurodeoxycholate (TDCA) and deoxycholate (DCA) on Lactobacillus reuteri CRL 1098, a strain of likely probiotic value. When compared to other lactobacilli, L. reuteri showed the highest survival rate but remained sensitive to high (>3 mM) DCA concentrations. DCA produced complete permeabilization of cells, abolished glucose uptake and severely distorted the cell envelope, as shown by electron microscopy. Detailed analytical studies revealed a change in the phospholipid to glycolipid ratio, and also in lipid proportions. The C18:1 W9C form remarkably increased, possibly following a rapid adaptive response during DCA treatment. This study provides the first solid evidence of the mechanism of DCA toxicity in a lactic acid bacterium.
María Pía Taranto; Gaspar Perez-Martinez; Graciela Font de Valdez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-02
Journal Detail:
Title:  Research in microbiology     Volume:  157     ISSN:  0923-2508     ISO Abbreviation:  Res. Microbiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907468     Medline TA:  Res Microbiol     Country:  France    
Other Details:
Languages:  eng     Pagination:  720-5     Citation Subset:  IM    
Centro de Referencia para Lactobacilos (CERELA), CONICET, Chacabuco 145, San Miguel de Tucumán, T4000ILC, Tucumán, Argentina. <>
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MeSH Terms
Cell Membrane / drug effects*,  ultrastructure
Cell Membrane Permeability / drug effects
Deoxycholic Acid / pharmacology*
Glucose / metabolism
Glycolipids / analysis
Lactobacillus / drug effects*,  physiology,  ultrastructure
Lactobacillus reuteri / drug effects,  physiology,  ultrastructure
Membrane Lipids / analysis
Microbial Viability / drug effects
Microscopy, Electron, Transmission
Phospholipids / analysis
Taurodeoxycholic Acid / pharmacology*
Reg. No./Substance:
0/Glycolipids; 0/Membrane Lipids; 0/Phospholipids; 50-99-7/Glucose; 516-50-7/Taurodeoxycholic Acid; 83-44-3/Deoxycholic Acid

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