Document Detail


Effect of betaine supplementation on changes in hepatic metabolism of sulfur-containing amino acids and experimental cholestasis induced by alpha-naphthylisothiocyanate.
MedLine Citation:
PMID:  15778005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations in the hepatic metabolism of sulfur amino acids in experimental cholestasis induced by alpha-naphthylisothiocyanate (ANIT) (100 mg/kg, po) were monitored in male mice for 1 week. We also examined the effects of betaine supplementation (1% in drinking water) for 2 weeks on the hepatotoxicity and changes in the sulfur amino acid metabolism induced by ANIT treatment. Acute ANIT challenge elevated the serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, and total bilirubin contents from 5 h after the treatment, reaching a peak at t = 48-72 h. Hepatic S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) levels were decreased significantly in a manner almost inversely proportional to the changes in serum parameters measured to determine the ANIT-induced toxicity. Hepatic glutathione and cysteine levels were elevated at t = 120 h after the treatment. Betaine supplementation blocked or significantly attenuated induction of the hepatotoxicity by ANIT. The decrease in SAM and SAH levels was also inhibited by betaine intake. The results indicate that betaine supplementation may antagonize the induction of experimental cholestasis and changes in the metabolism of sulfur amino acids associated with ANIT treatment. The underlying mechanism and pharmacological significance of its action are discussed.
Authors:
Young C Kim; Young S Jung; Sang K Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  43     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-03-21     Completed Date:  2005-04-25     Revised Date:  2006-03-13    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  663-70     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul 151-742, South Korea. youckim@snu.ac.kr
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MeSH Terms
Descriptor/Qualifier:
1-Naphthylisothiocyanate / toxicity
Alanine Transaminase / metabolism
Alkaline Phosphatase / metabolism
Amino Acids, Sulfur / metabolism*
Analysis of Variance
Animals
Aspartate Aminotransferases / metabolism
Betaine / administration & dosage,  pharmacology*
Bilirubin / metabolism
Cholestasis / chemically induced,  enzymology,  metabolism*
Cysteine / metabolism
Dietary Supplements
Glutathione / metabolism
Kinetics
Liver / drug effects,  enzymology,  metabolism*
Male
Mice
Mice, Inbred ICR
S-Adenosylhomocysteine / metabolism
S-Adenosylmethionine / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids, Sulfur; 107-43-7/Betaine; 29908-03-0/S-Adenosylmethionine; 52-90-4/Cysteine; 551-06-4/1-Naphthylisothiocyanate; 635-65-4/Bilirubin; 70-18-8/Glutathione; 979-92-0/S-Adenosylhomocysteine; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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