Document Detail

Effect of berberine on barrier function in a human retinal pigment epithelial cell line.
MedLine Citation:
PMID:  17295145     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1beta (IL-1beta). METHODS: ARPE-19 cells were cultured to confluence. Berberine and IL-1beta were added to the medium. Barrier functions were evaluated by measuring transepithelial electrical resistance (TER) and the permeability to horseradish peroxidase (HRP) and sodium fluorescein (SF). RESULTS: Berberine dose-dependently inhibited decreased TER and increased the permeability to HRP and SF in the cells stimulated with IL-1beta. CONCLUSIONS: Berberine dose-dependently inhibited the disruption of the barrier function in the ARPE-19 cell line induced by IL-1beta.
Hu-Shan Cui; Seiji Hayasaka; Xue-Yun Zhang; Yoriko Hayasaka; Zai-Long Chi; Lian-Shun Zheng
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Publication Detail:
Type:  Journal Article     Date:  2007-02-09
Journal Detail:
Title:  Japanese journal of ophthalmology     Volume:  51     ISSN:  0021-5155     ISO Abbreviation:  Jpn. J. Ophthalmol.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0044652     Medline TA:  Jpn J Ophthalmol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  64-7     Citation Subset:  IM    
Copyright Information:
(c) Japanese Ophthalmological Society 2007.
Department of Ophthalmology, University of Toyama, Toyama, Japan.
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MeSH Terms
Berberine / pharmacology*
Biological Transport / drug effects
Blood-Retinal Barrier / physiology*
Cell Line
Cell Membrane Permeability
Dose-Response Relationship, Drug
Electric Impedance
Fluorescein / metabolism
Horseradish Peroxidase / metabolism
Interleukin-1beta / pharmacology
Pigment Epithelium of Eye / drug effects*,  metabolism
Reg. No./Substance:
0/Interleukin-1beta; 2086-83-1/Berberine; 2321-07-5/Fluorescein; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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