Document Detail


Effect of atrial natriuretic peptide on ischemia-reperfusion injury in a porcine total hepatic vascular exclusion model.
MedLine Citation:
PMID:  17659696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To evaluate the effect of ANP on warm I/R injury in a porcine THVE model.
METHODS: Miniature pigs (mini-pigs) weighing 16-24 kg were observed for 120 min after reperfusion following 120 min of THVE. The animals were divided into two groups. ANP (0.1 mug/kg per min) was administered to the ANP group (n = 7), and vehicle was administered to the control group (n = 7). Either vehicle or ANP was intravenously administered from 30 min before the THVE to the end of the experiment. Arterial blood was collected to measure AST, LDH, and TNF-alpha. Hepatic tissue blood flow (HTBF) was also measured. Liver specimens were harvested for p38 MAPK analysis and histological study. Those results were compared between the two groups.
RESULTS: The AST and LDH levels were lower in the ANP group than in the control group; the AST levels were significantly different between the two groups (60 min: 568.7 +/- 113.3 vs 321.6 +/- 60.1, P = 0.038 < 0.05, 120 min: 673.6 +/- 148.2 vs 281.1 +/- 44.8, P = 0.004 < 0.01). No significant difference was observed in the TNF-alpha levels between the two groups. HTBF was higher in the ANP group, but the difference was not significant. A significantly higher level of phosphorylated p38 MAPK was observed in the ANP group compared to the control group (0 min: 2.92 +/- 1.1 vs 6.38 +/- 1.1, P = 0.011 < 0.05). Histological tissue damage was milder in the ANP group than in the control group.
CONCLUSION: Our results show that ANP has a protective role in I/R injury with p38 MAPK activation in a porcine THVE model.
Authors:
Katsumi Kobayashi; Kiyohiro Oshima; Masato Muraoka; Takahiko Akao; Osamu Totsuka; Hisashi Shimizu; Hiroaki Sato; Kazumi Tanaka; Kenjiro Konno; Koshi Matsumoto; Izumi Takeyoshi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  13     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-30     Completed Date:  2007-08-30     Revised Date:  2014-09-07    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3487-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine Transaminase / blood
Animals
Atrial Natriuretic Factor / therapeutic use*
Female
Ischemia / drug therapy*,  metabolism,  pathology
L-Lactate Dehydrogenase / blood
Liver / blood supply*
Male
Reperfusion Injury / prevention & control*
Swine
Tumor Necrosis Factor-alpha / blood
p38 Mitogen-Activated Protein Kinases / analysis,  physiology
Chemical
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 85637-73-6/Atrial Natriuretic Factor; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.6.1.2/Alanine Transaminase; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases
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