Document Detail


Effect of asymmetric dimethyl arginine on nitric oxide synthase activity in normal and pre-eclamptic placentae.
MedLine Citation:
PMID:  8743168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An endogenous inhibitor of nitric oxide synthase (NOS), NG,NG dimethylarginine (asymmetric dimethylarginine, ADMA), which is present in human plasma and urine, has been reported to be elevated in the plasma of women with pre-eclampsia. As ADMA inhibition may contribute to reduced placental NOS activity observed in pre-eclampsia, the aim of this study was to compare the effects of ADMA on placental NOS activity from pre-eclamptic and normal pregnancies (gestational ages 38.4 +/- 0.9 and 38.3 +/- 0.3 weeks respectively). NOS activity was determined by measuring the conversion of [3H]L-arginine to [3H]L-citrulline in homogenates of normal and pre-eclamptic placentae in the absence and presence of increasing concentrations of ADMA (1-100 microM). The IC50 for ADMA for the pre-eclamptic placentae (22.1 +/- 2.1 microM, n = 6) was not significantly different from that for the normal placentae (18.8 +/- 1.4 microM, n = 6). When ADMA and L-arginine in homogenates was removed by ion exchange chromatography and exogenous L-arginine replaced (32 microM), the IC50 for the pre-eclamptic placentae (19.5 +/- 1.8 microM, n = 6) was not significantly different than that for the normal placentae (20.9 +/- 1.0 microM, n = 6), and NOS activity in the absence of endogenous and exogenous ADMA was still reduced in pre-eclamptic placentae. These results provide no evidence that the sensitivity of placental NOS to ADMA is affected by pre-eclampsia, or that placental ADMA contributes to the reduction of placental NOS in pre-eclampsia.
Authors:
R G King; J L Di Iulio; N M Gude; S P Brennecke
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  7     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  1995  
Date Detail:
Created Date:  1996-09-13     Completed Date:  1996-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  1581-4     Citation Subset:  IM    
Affiliation:
Department of Perinatal Medicine, Royal Women's Hospital, Carlton, Vic., Australia.
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MeSH Terms
Descriptor/Qualifier:
Arginine / analogs & derivatives*,  pharmacology
Enzyme Inhibitors / pharmacology*
Female
Humans
Nitric Oxide Synthase / antagonists & inhibitors*
Placenta / drug effects,  enzymology*
Pre-Eclampsia / enzymology*
Pregnancy
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 30315-93-6/N,N-dimethylarginine; 74-79-3/Arginine; EC 1.14.13.39/Nitric Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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