Document Detail

Effect of ascorbic acid deficiency on primary and reparative dentinogenesis in non-ascorbate-synthesizing ODS rats.
MedLine Citation:
PMID:  9447259     Owner:  NLM     Status:  MEDLINE    
Ascorbic acid is essential to the biosynthesis of collagen, the major organic matrix component of dentine. The ODS rat is a mutant strain of Wistar rat characterized by hereditary lack of L-gulono-gamma-lactone oxidase and thus is unable to synthesize ascorbic acid. ODS rats were given an ascorbic acid-free diet to investigate how ascorbic acid deficiency affects dentine formation in vivo. Histomorphometric analysis on their growing molars and incisors showed a significant reduction in both size and mineral apposition rate of dentine, as revealed by contact microradiography and fluorescent time-marking, respectively. A similar reduction in bone formation was simultaneously demonstrated in the mandible, confirming the previously reported osteopathic effects of ascorbic acid deficiency. When pulp inflammation was induced in lower first molars by making unsealed pulp exposures, specimens from control animals showed continuous deposition of an osteodentine-like tissue in the radicular pulp chamber; this type of mineralized tissue formation was greatly reduced in ascorbic acid-deprived animals. These results indicate that ascorbic acid deficiency hampers dentine formation under both physiological and pathological conditions of the dentine/pulp complex. ODS rats could be useful in investigating in vivo effects of ascorbic acid deficiency on the formation of dentine and other dental mineralized tissues.
M Ogawara; K Aoki; T Okiji; H Suda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  42     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:    1997 Oct-Nov
Date Detail:
Created Date:  1998-03-03     Completed Date:  1998-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  695-704     Citation Subset:  D; IM    
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Ascorbic Acid / biosynthesis
Ascorbic Acid Deficiency / genetics,  physiopathology*
Collagen / biosynthesis
Dental Pulp Cavity / physiopathology
Dentin / metabolism,  physiopathology
Dentin, Secondary / metabolism,  physiopathology*
Dentinogenesis / physiology*
Fluorescent Dyes / diagnostic use
Incisor / pathology,  physiopathology
L-Gulonolactone Oxidase
Mandible / physiopathology
Metabolism, Inborn Errors / genetics
Minerals / metabolism
Molar / pathology,  physiopathology
Odontogenesis / physiology
Osteogenesis / physiology
Pulpitis / physiopathology
Rats, Mutant Strains
Rats, Wistar
Sugar Alcohol Dehydrogenases / deficiency
Reg. No./Substance:
0/Fluorescent Dyes; 0/Minerals; 50-81-7/Ascorbic Acid; 9007-34-5/Collagen; EC 1.1.-/Sugar Alcohol Dehydrogenases; EC Oxidase

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