| Effect of aroclor 1248 and two pure PCB congeners on phospholipase D activity in rat renal tubular cell cultures. | |
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MedLine Citation:
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PMID: 17427178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper elucidates the effect of different polychlorinated biphenyls (PCBs) on the phospholipase D (PLD) activity in soluble and particulate fractions of rat renal proximal tubular culture cells. Treatment with Aroclor 1248 (a commercial PCB mixture) caused a marked increase in the activity of PLD in intact renal tubular cells. The PLD activity was increased by Aroclor 1248 in the particulate fraction while the enzyme activity was unaffected in the soluble fraction. This work also shows that PCB 153 (2,2',4,4',5,5'-hexachlorobiphenyl, a di-ortho-substituted nonplanar congener) can increase the activity of PLD only in the particulate fraction. The exposure of cell cultures to PCB 77 (3,3',4,4'-tetrachlorobiphenyl, a non-ortho-substituted planar congener) does not alter PLD activity. These results suggest that PCB effects are structure dependent. Therefore, in order to clarify the molecular mechanism of activation of PLD by PCBs, the contents of immunoreactive PLD were examined by immunoblot analysis. Renal tubular cells expressed a PLD protein of 120 kDa corresponding with the PLD1 mammalian isoform in both the particulate and the soluble fraction. Aroclor 1248, PCB 153, and PCB 77 do not induce changes in the levels of PLD protein. These data indicate that PCBs, particularly nonplanar congeners, increase PLD activity. Moreover, these changes could not be demonstrated in the enzyme content in rat renal tubular cell cultures. |
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Authors:
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Mercedes Fernández Santiago; Pilar López-Aparicio; María N Recio; Miguel A Pérez-Albarsanz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and molecular toxicology Volume: 21 ISSN: 1095-6670 ISO Abbreviation: J. Biochem. Mol. Toxicol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-16 Completed Date: 2007-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9717231 Medline TA: J Biochem Mol Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 68-75 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aroclors / pharmacology* Cells, Cultured Environmental Pollutants / pharmacology* Gene Expression Regulation, Enzymologic / drug effects* Kidney Tubules, Proximal / enzymology* Phospholipase D / biosynthesis* Rats Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Aroclors; 0/Environmental Pollutants; 12672-29-6/aroclor 1248; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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