Document Detail

Effect of arachidonic acid on twitch tension of the rat phrenic nerve-diaphragm.
MedLine Citation:
PMID:  8450467     Owner:  NLM     Status:  MEDLINE    
Recent studies have demonstrated that unsaturated fatty acids are involved in the regulation of neuroeffector function. I have extended these studies by examining the effect of arachidonic acid on neuromuscular function in vitro using the rat phrenic nerve-diaphragm preparation. Arachidonic acid caused a time-and dose-dependent reduction in indirectly stimulated twitch tension, but had no effect on directly stimulated twitch tension. Linoleic acid and linolenic acid also reduced indirectly stimulated twitch tension, whereas stearic acid, oleic acid and arachidic acid had no effect. None of three blockers of arachidonic acid metabolism, the cyclooxygenase inhibitor indomethacin, the lipoxygenase inhibitor nordihydroguaiaretic acid or the cytochrome P-450 inhibitor ketoconazole, altered the effect of arachidonic acid on twitch tension. The free radical scavenger superoxide dismutase eliminated the inhibitory effect of arachidonic acid on twitch tension, suggesting that superoxide anion played a role in arachidonic acid's action.
R D Crosland
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  264     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-14     Completed Date:  1993-04-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1311-4     Citation Subset:  IM    
Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland.
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MeSH Terms
Arachidonic Acid / pharmacology*
Fatty Acids / pharmacology
Indomethacin / pharmacology
Neuromuscular Junction / drug effects*,  physiology
Rats, Sprague-Dawley
Superoxide Dismutase / pharmacology
Reg. No./Substance:
0/Fatty Acids; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; EC Dismutase

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