| Effect of arachidonic acid on [3H]D-aspartate outflow in the rat hippocampus. | |
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MedLine Citation:
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PMID: 8183429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the effect of arachidonic acid on [3H]d-aspartate outflow in rat hippocampus synaptosomes and slices. Arachidonic acid 1) increased basal outflow of [3H]d-aspartate in both synaptosomes and slices, and 2) increased K(+)-evoked overflow in slices but not in synaptosomes. The latter effect was dependent (at least in part) on arachidonic acid metabolism, most likely mediated by lipo-oxygenase metabolites and free radical production. It was prevented by nordihydroguairetic acid but not by indomethacin, and was significantly reduced by free radical scavengers (superoxide-dismutase and catalase). This effect was dependent upon stimulation since it could not be observed after a continuous perfusion of arachidonic acid in the absence of stimulation. Furthermore, it was long-lasting since a 30 min perfusion of arachidonic acid was sufficient to exert a significant effect on a stimulation following termination of the application. |
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Authors:
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M Simonato; G Bregola; C Bianchi; L Beani |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurochemical research Volume: 19 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-06-14 Completed Date: 1994-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 195-200 Citation Subset: IM |
Affiliation:
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Institute of Pharmacology, University of Ferrara, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / pharmacology* Aspartic Acid / metabolism* Basal Metabolism Biological Markers / chemistry Free Radicals Glutamates / metabolism* Hippocampus / drug effects*, metabolism Lipoxygenase / metabolism Male Potassium / pharmacology Rats Rats, Sprague-Dawley Synaptosomes / drug effects, metabolism Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Free Radicals; 0/Glutamates; 10028-17-8/Tritium; 506-32-1/Arachidonic Acid; 56-84-8/Aspartic Acid; 7440-09-7/Potassium; EC 1.13.11.12/Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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