Document Detail

Effect of apolipoprotein E on cell viability in a human neuroblastoma cell line: influence of oxidation and lipid-association.
MedLine Citation:
PMID:  10806314     Owner:  NLM     Status:  MEDLINE    
Apolipoprotein E (apoE) whose polymorphic expression is widely associated with Alzheimer's disease (AD) is one of the most studied protein present in cerebral amyloid deposits. Native or fragments of apoE are known to exert neurotoxic effects. We evaluated the effects of apoE oxidation and lipid-association on the viability of human neuroblastoma IMR32 cells. We show that apoE affects cell viability only when it is lipid-associated and applied at a concentration near to that found in plasma, and this whatever the isoform. Oxidized phospholipid-associated apoE has a similar impact on cell viability. These findings show the necessity of including apoE into phospholipids when studying its effect on cell metabolism and underline the probable intervention of surface heparan sulfate proteoglycans (HSPG). It also warrants further studies in order to delineate the pathophysiological importance of apoE.
A Hoy; B Leininger-Muller; C Jolivalt; G Siest
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  285     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-01     Completed Date:  2000-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  173-6     Citation Subset:  IM    
Centre du Médicament, Université Henri Poincaré Nancy I, 30 rue Lionnois, 54000, Nancy, France.
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MeSH Terms
Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Apolipoproteins E / cerebrospinal fluid,  pharmacology*
Cell Survival / drug effects
Phospholipids / pharmacology*
Protein Isoforms / pharmacology
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Apolipoprotein E2; 0/Apolipoprotein E3; 0/Apolipoprotein E4; 0/Apolipoproteins E; 0/Phospholipids; 0/Protein Isoforms

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