Document Detail


Effect of apolipoprotein E on cell viability in a human neuroblastoma cell line: influence of oxidation and lipid-association.
MedLine Citation:
PMID:  10806314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apolipoprotein E (apoE) whose polymorphic expression is widely associated with Alzheimer's disease (AD) is one of the most studied protein present in cerebral amyloid deposits. Native or fragments of apoE are known to exert neurotoxic effects. We evaluated the effects of apoE oxidation and lipid-association on the viability of human neuroblastoma IMR32 cells. We show that apoE affects cell viability only when it is lipid-associated and applied at a concentration near to that found in plasma, and this whatever the isoform. Oxidized phospholipid-associated apoE has a similar impact on cell viability. These findings show the necessity of including apoE into phospholipids when studying its effect on cell metabolism and underline the probable intervention of surface heparan sulfate proteoglycans (HSPG). It also warrants further studies in order to delineate the pathophysiological importance of apoE.
Authors:
A Hoy; B Leininger-Muller; C Jolivalt; G Siest
Related Documents :
3071794 - Aberration in de novo ether lipid biosynthesis in peroxisomal disorders.
12168034 - Metabolism of oxidized high density lipoprotein-2(ox-hdl(2)) in rat hepatic sinusoidal ...
2920014 - Uptake of retinyl ester in hl-60 cells via the low-density-lipoprotein-receptor pathway.
20336284 - Membrane organization and regulation of cellular cholesterol homeostasis.
1348694 - Annexin i and involucrin are cross-linked by particulate transglutaminase into the corn...
22193304 - Comprehensive qpcr profiling of gene expression in single neuronal cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  285     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-01     Completed Date:  2000-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  173-6     Citation Subset:  IM    
Affiliation:
Centre du Médicament, Université Henri Poincaré Nancy I, 30 rue Lionnois, 54000, Nancy, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Apolipoproteins E / cerebrospinal fluid,  pharmacology*
Cell Survival / drug effects
Humans
Neuroblastoma
Oxidation-Reduction
Phospholipids / pharmacology*
Protein Isoforms / pharmacology
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Apolipoprotein E2; 0/Apolipoprotein E3; 0/Apolipoprotein E4; 0/Apolipoproteins E; 0/Phospholipids; 0/Protein Isoforms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Vagotomy does not inhibit high dose lipopolysaccharide-induced interleukin-1beta immunoreactivity in...
Next Document:  Differential sensitivity of rat hippocampal and cortical astrocytes to oxygen-glucose deprivation in...