Document Detail

Effect of antispermatogenic agents on cell marker enzymes of rat Sertoli cells in vitro.
MedLine Citation:
PMID:  16371305     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine the role of Sertoli cells in the antispermatogenic action of two nonsteroidal male contraceptive compounds (CDRI-84/35 and gossypol) by evaluating their effect on some key parameters of Sertoli cell function in vitro. METHODS: Primary cultures of Sertoli cell were established from 18-day-old rat testis and treated on day 5 with different concentrations (1.0, 0.1, 0.01, and 0.001 mM) of either CDRI-84/35 or gossypol in vitro. Lactate (secretion), along with beta-glucuronidase, gamma-glutamyl transpeptidase, lactate dehydrogenase (LDH) and aromatase activities, was measured in these cells to examine the functions targeted by antispermatogenic agents in Sertoli cells. RESULTS: CDRI-84/35 significantly affected Sertoli cell parameters (stimulation in most of the cases) that are important for germ cell development like lactate secretion, LDH activity, aromatase activity (estradiol secretion) and so on. Gossypol in comparison to CDRI-84/35 had a more severe effect on Sertoli cells with complete inhibition of enzyme activities at higher concentrations. CONCLUSION: It is probable that the antispermatogenic action of CDRI-84/35 and gossypol is routed through Sertoli cells by disruption of important cell functions that support spermatogenesis in vivo. However, the two compounds appear to have different course of action in Sertoli cells, ultimately leading to spermatogenic failure.
Priti Ojha; Janak Dulari Dhar; Anil Kumar Dwivedi; Ram Lakhan Singh; Gopal Gupta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-14
Journal Detail:
Title:  Contraception     Volume:  73     ISSN:  0010-7824     ISO Abbreviation:  Contraception     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-22     Completed Date:  2006-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0234361     Medline TA:  Contraception     Country:  United States    
Other Details:
Languages:  eng     Pagination:  102-6     Citation Subset:  IM    
Division of Endocrinology, Central Drug Research Institute, Lucknow-226 001, India.
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MeSH Terms
Antispermatogenic Agents / pharmacology*
Aromatase / metabolism
Cells, Cultured
Glucuronidase / metabolism
Gossypol / pharmacology
L-Lactate Dehydrogenase / metabolism
Lactic Acid / metabolism
Piperazines / pharmacology
Rats, Sprague-Dawley
Sertoli Cells / drug effects*,  enzymology*
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
0/1-formyl-4-dichloroacetylpiperazine; 0/Antispermatogenic Agents; 0/Piperazines; 303-45-7/Gossypol; 50-21-5/Lactic Acid; EC Dehydrogenase; EC; EC; EC

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