Document Detail

Effect of antisense oligodeoxynucleotide for sepiapterin reductase on the viability of PC12 cells in the presence of exogenous carbonyl compounds.
MedLine Citation:
PMID:  12604243     Owner:  NLM     Status:  MEDLINE    
Sepiapterin reductase (SPR) is known as an essential enzyme for the biosynthesis of tetrahydrobiopterin. SPR belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. We found in the present study that phenylpropanedione decreased the rate of proliferation of PC12 cells and that this rate was further diminished by the transfection of the cells with antisense oligodeoxynucleotide for SPR mRNA. When the cells were treated with N-acetylserotonin, a specific inhibitor of SPR, in the presence of phenylpropanedione, the cell number decreased to almost the same level as when the cells were transfected with the antisense oligodeoxynucleotide. Thus, the SDR activity of SPR in PC12 cells may serve for detoxification of exogenous carbonyl compounds besides functioning as a specific enzyme for the formation of tetrahydrobiopterin.
Kengo Fujimoto; Masato Sakurai; Masami Kawase; Setsuko Katoh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemico-biological interactions     Volume:  143-144     ISSN:  0009-2797     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-26     Completed Date:  2003-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  583-6     Citation Subset:  IM    
Department of Biochemistry, Meikai University School of Dentistry, Sakado, 350-0283, Saitama, Japan.
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MeSH Terms
Alcohol Oxidoreductases / genetics*,  metabolism
Cell Survival / genetics
Oligonucleotides, Antisense / pharmacology*
PC12 Cells
RNA, Messenger / genetics
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/RNA, Messenger; EC 1.1.-/Alcohol Oxidoreductases; EC reductase

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