Document Detail

Effect of antagonists of calcium and phospholipase A on the cytopathogenicity of Entamoeba histolytica.
MedLine Citation:
PMID:  2863317     Owner:  NLM     Status:  MEDLINE    
The in vitro mechanisms by which Entamoeba histolytica trophozoites lyse target Chinese hamster ovary (CHO) cells were examined. Calcium chelators ethylenediaminetetraacetate and ethyleneglycol bis (beta-aminoethyl ether)-N,N'-tetraacetate (10 mM) inhibited amebic cytolysis of target CHO cells (P less than .01). A putative antagonist of intracellular calcium flux, 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8; greater than or equal to 250 microM), inhibited amebic adherence and cytolysis (P less than .001). Quinacrine, Rosenthal's inhibitor (dimethyl-dl-2,3-distearoyloxypropyl-2'-hydroxyethyl ammonium acetate), phosphatidylcholine, and hydrocortisone (greater than or equal to 10(-4) M), all pharmacological antagonists of eukaryotic phospholipase A enzymes, inhibited amebic killing of target CHO cells (P less than .001). At 37 C quinacrine and hydrocortisone reduced amebic adherence to CHO cells, whereas Rosenthal's inhibitor and phosphatidylcholine did not. Phosphatidylcholine and TMB-8 demonstrated a synergistic inhibitory effect on amebic killing of target CHO cells (P less than .001). These studies indicate that extracellular calcium ions, amebic intracellular calcium flux, and amebic phospholipase A activity are required for cytolysis of target cells by E. histolytica.
J I Ravdin; C F Murphy; R L Guerrant; S A Long-Krug
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  152     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-10-10     Completed Date:  1985-10-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  542-9     Citation Subset:  AIM; IM    
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MeSH Terms
Calcium / antagonists & inhibitors,  metabolism*
Calcium Channel Blockers / pharmacology
Cell Line
Cell Survival
Edetic Acid / pharmacology
Entamoeba histolytica / enzymology,  metabolism,  pathogenicity*
Gallic Acid / analogs & derivatives,  pharmacology
Hydrocortisone / pharmacology
Phosphatidylcholines / pharmacology
Phospholipases / metabolism*
Phospholipases A / antagonists & inhibitors,  metabolism*
Quinacrine / pharmacology
Stearates / pharmacology
Grant Support
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Phosphatidylcholines; 0/Stearates; 149-91-7/Gallic Acid; 50-23-7/Hydrocortisone; 57818-92-5/8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; 60-00-4/Edetic Acid; 7059-39-4/Rosenthal's inhibitor; 7440-70-2/Calcium; 83-89-6/Quinacrine; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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