Document Detail

Effect of anaerobiosis and glucose on the content of haem and its precursors in intact yeast cells.
MedLine Citation:
PMID:  506615     Owner:  NLM     Status:  MEDLINE    
The content of haem and its precursors was determined in yeast cells grown under various conditions. The cells grown aerobically on 2% galactose contain about three times more haem (about 300 nmoles/g dry wt.) than the cells grown on 10% glucose. A trace amount of haem was found in anoxia irrespective of the carbon source used. The "efficiency" of the first enzyme of the haem biosynthetic pathway--delta-aminolevulinic acid (ALA) synthetase--expressed as the sum of all intermediates of the pathway in the cells grown on galactose, is similar in anaerobic and aerobic cells. The "efficiency" of the second enzyme--ALA dehydratase--is lower about three times both in anoxia and under conditions of glucose repression. In anoxia, not haem but delta-aminolevulinic acid is the main biosynthetic product. The role of glucose repression and of the feedback mechanisms in regulation of haem synthesis in yeast is discussed. A method for haem determination in the intact yeast cells, based on the formation of pyridine haemochrome, is described.
J Lukaszkiewicz; T Biliński
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  26     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  1979  
Date Detail:
Created Date:  1980-01-24     Completed Date:  1980-01-24     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  161-9     Citation Subset:  IM    
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MeSH Terms
5-Aminolevulinate Synthetase / metabolism
Aminolevulinic Acid
Culture Media
Glucose / pharmacology
Heme / biosynthesis*
Porphobilinogen Synthase / metabolism
Yeasts / cytology,  growth & development,  metabolism*
Reg. No./Substance:
0/Culture Media; 106-60-5/Aminolevulinic Acid; 14875-96-8/Heme; 50-99-7/Glucose; EC Synthetase; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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