| Effect of anaerobiosis and glucose on the content of haem and its precursors in intact yeast cells. | |
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MedLine Citation:
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PMID: 506615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The content of haem and its precursors was determined in yeast cells grown under various conditions. The cells grown aerobically on 2% galactose contain about three times more haem (about 300 nmoles/g dry wt.) than the cells grown on 10% glucose. A trace amount of haem was found in anoxia irrespective of the carbon source used. The "efficiency" of the first enzyme of the haem biosynthetic pathway--delta-aminolevulinic acid (ALA) synthetase--expressed as the sum of all intermediates of the pathway in the cells grown on galactose, is similar in anaerobic and aerobic cells. The "efficiency" of the second enzyme--ALA dehydratase--is lower about three times both in anoxia and under conditions of glucose repression. In anoxia, not haem but delta-aminolevulinic acid is the main biosynthetic product. The role of glucose repression and of the feedback mechanisms in regulation of haem synthesis in yeast is discussed. A method for haem determination in the intact yeast cells, based on the formation of pyridine haemochrome, is described. |
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Authors:
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J Lukaszkiewicz; T Biliński |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 26 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 1979 |
Date Detail:
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Created Date: 1980-01-24 Completed Date: 1980-01-24 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: POLAND |
Other Details:
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Languages: eng Pagination: 161-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Aminolevulinate Synthetase
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metabolism Aminolevulinic Acid Anaerobiosis Culture Media Glucose / pharmacology Heme / biosynthesis* Porphobilinogen Synthase / metabolism Yeasts / cytology, growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 106-60-5/Aminolevulinic Acid; 14875-96-8/Heme; 50-99-7/Glucose; EC 2.3.1.37/5-Aminolevulinate Synthetase; EC 4.2.1.24/Porphobilinogen Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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