Document Detail


Effect of amphotercin B on urine acidification in rats: implications for the pathogenesis of distal renal tubular acidosis.
MedLine Citation:
PMID:  839105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been proposed that distal renal tubular acidosis is a gradient-limited disorder an that the low urine Pco2 observed in this condition is caused by back diffusion of carbonic acid. This study was designed to examine this hypothesis using the amphotericin B model of gradient-limited distal renal tubular acidosis in rats. After induction of acute metabolic acidosis the minimum urine pH in 12 of 24 amphotericin B-treated rats exceeded 5.63 (mean 5.76 +/- 0.04), whereas it was 5.41 +/-0.04 in control rats. These animals with impaired urine acidification were presumed to have a gradient lesion and were studied in bicarbonate-loading experiments. The urine minus blood Pco2 gradient in these rats was 24.9 +/- 1.5 mm. Hg, a value similar to that of the control rats (26.7 +/- 2.1 mm. Hg). The presence of a normal urine minus blood Pco2 value in this experimentally induced gradient-limited type of acidification lesion indicates that a permeability defect for hydrogen ions was not associated with a similar defect for carbonic acid and that the urine minus blood Pco2 gradient is a valid index of distal nephron hydrogen ion secretion in amphotericin B-like gradient-type lesions.
Authors:
J M Roscoe; M B Goldstein; M L Halperin; F X Schloeder; B J Stinebaugh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  89     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-04-30     Completed Date:  1977-04-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  463-70     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Acidosis, Renal Tubular / chemically induced,  etiology*,  urine
Amphotericin B
Animals
Carbon Dioxide / urine
Furosemide / pharmacology
Kidney / metabolism
Kidney Concentrating Ability
Male
Osmolar Concentration
Rats
Chemical
Reg. No./Substance:
124-38-9/Carbon Dioxide; 1397-89-3/Amphotericin B; 54-31-9/Furosemide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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