| Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry. | |
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MedLine Citation:
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PMID: 15047545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment. |
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Authors:
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Marie Claude Barc; François Bourlioux; Lionel Rigottier-Gois; Céline Charrin-Sarnel; Claire Janoir; Hélène Boureau; Joël Doré; Anne Collignon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Antimicrobial agents and chemotherapy Volume: 48 ISSN: 0066-4804 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-29 Completed Date: 2004-05-24 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: United States |
Other Details:
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Languages: eng Pagination: 1365-8 Citation Subset: IM |
Affiliation:
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Unité associée INRA Ecosystème Microbien Digestif et Santé, Faculté de Pharmacie, Université Paris-Sud, 92296 Châtenay-Malabry Cedex, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amoxicillin-Potassium Clavulanate Combination
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pharmacology* Animals Bacteria, Anaerobic / drug effects Colony Count, Microbial Drug Therapy, Combination / pharmacology* Feces / microbiology* Female Flow Cytometry Germ-Free Life Humans In Situ Hybridization, Fluorescence Mice Mice, Inbred C3H Oligonucleotide Probes RNA Probes / diagnostic use RNA, Ribosomal, 16S / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotide Probes; 0/RNA Probes; 0/RNA, Ribosomal, 16S; 74469-00-4/Amoxicillin-Potassium Clavulanate Combination |
| Comments/Corrections | |
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