| Effect of amino acids on aggregation behaviour of sodium deoxycholate in solution: a fluorescence study. | |
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MedLine Citation:
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PMID: 21608102 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The influence of three alkaline amino acids, l-lysine (L-Lys), l-arginine (L-Arg) and l-histidine (L-His), on the aggregation behaviour of sodium deoxycholate (NaDC) in phosphate buffer, pH 7.0, was studied at 25 °C. The fluorescence probe technique based on pyrene was employed to determine accurately the critical aggregation concentration (cac), polarity of the microenvironment and aggregation numbers for the NaDC aggregates. The added amino acids can effectively reduce the cac values and micropolarity of NaDC, indicating that it is easier for NaDC to aggregate in a compact manner in the presence of amino acids. The aggregation numbers of NaDC were increased, indicating that more NaDC molecules connect together to form stable aggregates. The performance of L-Arg is similar to that of L-His, and both have a smaller effect on the above parameters than L-Lys. In view of this, it may be inferred that both electrostatic and hydrophobic interaction are responsible for the interaction between NaDC and amino acids in aqueous solution. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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Fang He; Guiying Xu; Jinyu Pang; Tingting Han; Teng Liu; Xiaodeng Yang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-24 |
Journal Detail:
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Title: Luminescence : the journal of biological and chemical luminescence Volume: - ISSN: 1522-7243 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100889025 Medline TA: Luminescence Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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