| Effect of amino acid levels on matrix vesicle formation by epiphyseal growth plate chondrocytes in primary culture. | |
| | |
MedLine Citation:
|
PMID: 3949889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of varying the amino acid concentrations of the culture medium on matrix vesicle formation was studied in primary cultures of chicken epiphyseal growth plate chondrocytes grown in Dulbecco's modified Eagle's medium (DME) supplemented with 10% fetal bovine serum (FBS). Decreasing the levels of free amino acids in the culture medium to levels of one-half, one quarter, and one eighth of the values normally present in DME caused a progressive decline in matrix vesicle (MV) formation. Increasing the level in the culture medium of those amino acids that are enriched in extracellular fluid (ECF) of growth plate cartilage significantly increased formation of matrix vesicles (MV), as assayed by the alkaline phosphatase (AP) activities present in high-speed sediments from spent culture media. However, adjusting the levels of all amino acids to match those of the ECF produced the greatest stimulation of MV formation. Of the amino acids that are notably enriched in ECF, glutamate (GLU), alanine (ALA), serine (SER), asparagine (ASN), and taurine (TAU) individually enhanced MV production, whereas proline (PRO), glycine (GLY), and aspartate (ASP) had essentially no effect. The simple combination of ECF levels of ALA and GLU resulted in a stimulation of MV formation equal to that observed when the eight aforementioned amino acids were elevated to ECF levels. Other combinations of ASP and GLY, or of TAU, SER, and ASN showed some stimulation, but at a lower level. Increasing the amino acid concentrations, alone or in combination, also increased the levels of cellular AP, and to a lesser extent cellular protein. While increases in cellular AP were generally correlated with increased formation of AP-rich MV, this was not uniformly true. These results indicate that in addition to hormones and growth factors, nutritional factors such as the levels of amino acids are also critical for normal phenotypic expression, growth, and matrix formation by epiphyseal chondrocytes. |
| | |
Authors:
|
Y Ishikawa; J E Chin; E M Schalk; R E Wuthier |
Related Documents
:
|
22021809 - Technical note: stearidonic acid metabolism by mixed ruminal microorganisms in vitro. 19187129 - Sphagnan--a pectin-like polymer isolated from sphagnum moss can inhibit the growth of s... 129479 - The influence of gibberellic acid and abscisic acid on cell and tissue differentiation ... 16094969 - The influence of glutamic acid and arginine on the competence of bacillus subtilis grow... 4988249 - Complexing of fatty acids by triton wr1339 in relation to growth of mycobacterium tuber... 20925679 - Growth regulators in populus tremula i. distribution of auxin and growth inhibitors. 16435489 - Acid secretory potency and elimination of the 15-leucine gastrin-17 analogue in man. 5441549 - Mechanism of excessive purine biosynthesis in hypoxanthine-guanine phosphoribosyltransf... 7872779 - Biochemical characterization of lauric acid omega-hydroxylation by a cyp4a1/nadph-cytoc... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 126 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1986 Mar |
Date Detail:
|
Created Date: 1986-04-03 Completed Date: 1986-04-03 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 399-406 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis* Animals Cartilage / cytology Cells, Cultured Chickens Culture Media Epiphyses / cytology*, metabolism Extracellular Matrix / metabolism, ultrastructure* |
| Grant Support | |
ID/Acronym/Agency:
|
AM 18983/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of retinoic acid on the ultrastructure and hyaluronic acid synthesis of adult human epider...
Next Document: Increased free calcium in endothelial cells under stimulation with adenine nucleotides.