| Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri. | |
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MedLine Citation:
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PMID: 19376900 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B(12) production and provided new avenues for future improvement of production of vitamin B(12). |
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Authors:
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Filipe Santos; Bas Teusink; Douwe Molenaar; Maurice van Heck; Michiel Wels; Sander Sieuwerts; Willem M de Vos; Jeroen Hugenholtz |
Publication Detail:
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Type: Journal Article Date: 2009-04-17 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 75 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-09 Completed Date: 2009-06-25 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3930-6 Citation Subset: IM |
Affiliation:
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Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, BA Ede, The Netherlands. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GPL7505; GSE13391 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Culture Media / chemistry Gene Expression Profiling Lactobacillus reuteri / metabolism* Oligonucleotide Array Sequence Analysis Vitamin B 12 / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 68-19-9/Vitamin B 12 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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