| Effect of ambient temperature on in vitro fertilization of Bubaline oocyte. | |
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MedLine Citation:
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PMID: 17125942 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was used to examine the effect of ambient temperature on the day of slaughter of buffaloes on oocyte cleavage and subsequent embryo development following in vitro fertilization (IVF)/chemical activation (parthenogenesis). A total of 601 oocytes were collected from buffaloes, which were sacrificed when the ambient temperature was >40 or < or =40 degrees C and the collected oocytes were matured in vitro. During each experiment about half of the matured oocytes were used for IVF whereas the remaining oocytes were subjected to one of the three chemical activation protocols viz. (i) 7% ethanol (ET) and 6-di methyl amino purine (6-DMAP), (ii) ET and cycloheximide (CHX) and (iii) ET followed by a combined treatment of 6-DMAP and CHX. Cleaved oocytes were cultured in mSOF supplemented with BSA, essential amino acids, non-essential amino acids, ITS (insulin transferrin and selenium) and L-glutamine. Low cleavage and subsequent embryo development was observed in those oocytes which were collected from buffaloes slaughtered at ambient temperature >40 degrees C than at < or =40 degrees C. There was no significant difference in cleavage rate following different chemical activations in oocytes collected from buffaloes slaughtered on the day when the maximum ambient temperature was >40 degrees C or < or =40 degrees C. These results suggest that high ambient temperature influences competence of oocytes to cleave and develop to blastocyst stage following natural activation with sperm and/or process of fertilization and subsequent embryo development. |
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Authors:
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V Mishra; A K Misra; R Sharma |
Publication Detail:
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Type: Journal Article Date: 2006-10-25 |
Journal Detail:
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Title: Animal reproduction science Volume: 100 ISSN: 0378-4320 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-05-28 Completed Date: 2007-08-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 379-84 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, GB Pant University of Agriculture and Technology, Pantnagar-263 145, Udham Singh Nagar, Uttaranchal, India. mishrav@30gigs.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives,
pharmacology Animals Buffaloes / physiology* Cell Culture Techniques Cycloheximide / pharmacology Ethanol / pharmacology Female Fertilization in Vitro / methods, veterinary* Oocytes / drug effects, physiology* Temperature |
| Chemical | |
Reg. No./Substance:
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64-17-5/Ethanol; 66-81-9/Cycloheximide; 73-24-5/Adenine; 938-55-6/N(6),N(6)-dimethyladenine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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